Validation Data Gallery
|Positive WB detected in||HeLa cells, HepG2 cells, Jurkat cells, mouse liver tissue, rat liver tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17776-1-AP targets VPS16 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VPS16 fusion protein Ag12202|
|Full Name||vacuolar protein sorting 16 homolog (S. cerevisiae)|
|Calculated molecular weight||839 aa, 95 kDa|
|Observed molecular weight||95 kDa|
|GenBank accession number||BC073959|
|Gene ID (NCBI)||64601|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. Human VPS16 is the homolog of yeast class C Vps16 protein. VPS16 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
Biochim Biophys Acta Mol Cell Res
Lysosomal regulation of extracellular vesicle excretion during d-ribose-induced NLRP3 inflammasome activation in podocytes.
Proteomics Clin Appl
Label-Free Quantitative Proteomic Profiling of LAD2 Mast Cell Releasates Reveals the Mechanism of Tween-80-Induced Anaphylactoid Reaction.
Biochem Biophys Res Commun
Overexpression of VPS16 correlates with tumor progression and chemoresistance in colorectal cancer.
Am J Physiol Cell Physiol
Control of Lysosomal TRPML1 Channel Activity and Exosome Release by Acid Ceramidase in Mouse Podocytes.
Biochim Biophys Acta Mol Basis Dis
Contribution of podocyte inflammatory exosome release to glomerular inflammation and sclerosis during hyperhomocysteinemia.
Biochim Biophys Acta Mol Cell Biol Lipids
Abnormal podocyte TRPML1 channel activity and exosome release in mice with podocyte-specific Asah1 gene deletion.