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- KD/KO Validated
VPS16 Polyclonal antibody
VPS16 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, ELISA
Cat no : 17776-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, HepG2 cells, Jurkat cells, mouse liver tissue, rat liver tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 3 publications below|
|WB||See 10 publications below|
|IF||See 8 publications below|
17776-1-AP targets VPS16 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VPS16 fusion protein Ag12202|
|Full Name||vacuolar protein sorting 16 homolog (S. cerevisiae)|
|Calculated molecular weight||839 aa, 95 kDa|
|Observed molecular weight||95 kDa|
|GenBank accession number||BC073959|
|Gene ID (NCBI)||64601|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. Human VPS16 is the homolog of yeast class C Vps16 protein. VPS16 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
|Product Specific Protocols|
|WB protocol for VPS16 antibody 17776-1-AP||Download protocol|
|IHC protocol for VPS16 antibody 17776-1-AP||Download protocol|
|IP protocol for VPS16 antibody 17776-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Distinct autophagosomal-lysosomal fusion mechanism revealed by thapsigargin-induced autophagy arrest.
SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes
Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.
Regulation of TRPML1 channel activity and inflammatory exosome release by endogenously produced reactive oxygen species in mouse podocytes.
Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.
Cell Death Dis
Autophagosome maturation mediated by Rab7 contributes to neuroprotection of hypoxic preconditioning against global cerebral ischemia in rats.