• Featured Product
  • KD/KO Validated

VPS16 Polyclonal antibody

VPS16 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 17776-1-AP


hVPS16, VPS16

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17776-1-AP targets VPS16 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS16 fusion protein Ag12202
Full Name vacuolar protein sorting 16 homolog (S. cerevisiae)
Calculated Molecular Weight 839 aa, 95 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC073959
Gene Symbol VPS16
Gene ID (NCBI) 64601
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. Human VPS16 is the homolog of yeast class C Vps16 protein. VPS16 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.


Product Specific Protocols
WB protocol for VPS16 antibody 17776-1-APDownload protocol
IHC protocol for VPS16 antibody 17776-1-APDownload protocol
IP protocol for VPS16 antibody 17776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy

Authors - Shen Zhang

Mol Cell

Distinct autophagosomal-lysosomal fusion mechanism revealed by thapsigargin-induced autophagy arrest.

Authors - Ian G Ganley
  • KD Validated

J Hazard Mater

VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes

Authors - Tao Wang


SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng


Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.

Authors - Qiuhong Shen

Redox Biol

Regulation of TRPML1 channel activity and inflammatory exosome release by endogenously produced reactive oxygen species in mouse podocytes.

Authors - Guangbi Li