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  • KD/KO Validated

VPS16 Polyclonal antibody

VPS16 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 17776-1-AP


hVPS16, VPS16

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17776-1-AP targets VPS16 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS16 fusion protein Ag12202
Full Name vacuolar protein sorting 16 homolog (S. cerevisiae)
Calculated molecular weight 839 aa, 95 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC073959
Gene symbol VPS16
Gene ID (NCBI) 64601
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. Human VPS16 is the homolog of yeast class C Vps16 protein. VPS16 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.


Product Specific Protocols
WB protocol for VPS16 antibody 17776-1-APDownload protocol
IHC protocol for VPS16 antibody 17776-1-APDownload protocol
IP protocol for VPS16 antibody 17776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochim Biophys Acta Mol Cell Res

Lysosomal regulation of extracellular vesicle excretion during d-ribose-induced NLRP3 inflammasome activation in podocytes.

Authors - Jinni Hong

Proteomics Clin Appl

Label-Free Quantitative Proteomic Profiling of LAD2 Mast Cell Releasates Reveals the Mechanism of Tween-80-Induced Anaphylactoid Reaction.

Authors - Yan-Ni Mi

Biochem Biophys Res Commun

Overexpression of VPS16 correlates with tumor progression and chemoresistance in colorectal cancer.

Authors - Baoqing Zhang
  • KD Validated

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Control of Lysosomal TRPML1 Channel Activity and Exosome Release by Acid Ceramidase in Mouse Podocytes.

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Contribution of podocyte inflammatory exosome release to glomerular inflammation and sclerosis during hyperhomocysteinemia.

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Abnormal podocyte TRPML1 channel activity and exosome release in mice with podocyte-specific Asah1 gene deletion.

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