VPS16 Polyclonal antibody

• Featured Product
• KD/KO Validated

VPS16 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 17776-1-AP

Synonyms

hVPS16, VPS16

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Validation Data Gallery

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  WB analysis using 17776-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17776-1-AP (VPS16 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17776-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17776-1-AP (VPS16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17776-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17776-1-AP (VPS16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17776-1-AP

  IP Result of anti-VPS16 (IP:17776-1-AP, 3ug; Detection:17776-1-AP 1:500) with HeLa cells lysate 2480ug.

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  IHC staining of human liver using 17776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17776-1-AP (VPS16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17776-1-AP (VPS16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17776-1-AP (VPS16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17776-1-AP (VPS16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IF: See 8 publications below

Product Information

17776-1-AP targets VPS16 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS16 fusion protein Ag12202
• Full Name: vacuolar protein sorting 16 homolog (S. cerevisiae)
• Calculated molecular weight: 839 aa, 95 kDa
• Observed molecular weight: 95 kDa
• GenBank accession number: BC073959
• Gene symbol: VPS16
• Gene ID (NCBI): 64601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. Human VPS16 is the homolog of yeast class C Vps16 protein. VPS16 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Distinct autophagosomal-lysosomal fusion mechanism revealed by thapsigargin-induced autophagy arrest.; Authors - Ian G Ganley ; KD Validated
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB	Autophagy; Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.; Authors - Qiuhong Shen
mouse	IF	Redox Biol; Regulation of TRPML1 channel activity and inflammatory exosome release by endogenously produced reactive oxygen species in mouse podocytes.; Authors - Guangbi Li
human	WB	Curr Biol; Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.; Authors - Anna Ulbricht
rat	WB	Cell Death Dis; Autophagosome maturation mediated by Rab7 contributes to neuroprotection of hypoxic preconditioning against global cerebral ischemia in rats.; Authors - Lixuan Zhan