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C15orf58 Polyclonal antibody

C15orf58 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25447-1-AP

Synonyms

C15orf58, UPF0580 protein C15orf58

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using C15orf58 Polyclonal antibody (25447-1-AP)

    WB analysis of PC-3 using 25447-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25447-1-AP (C15orf58 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using C15orf58 Polyclonal antibody (25447-1-AP)

    WB analysis of mouse brain using 25447-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25447-1-AP (C15orf58 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using C15orf58 Polyclonal antibody (25447-1-AP)

    WB analysis of PC-3 using 25447-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25447-1-AP (C15orf58 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using C15orf58 Polyclonal antibody (25447-1-AP)

    IF Staining of PC-3 using 25447-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 25447-1-AP (C15orf58 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells, mouse brain tissue
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25447-1-AP is C15orf58 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C15orf58 fusion protein Ag20215
Full Name chromosome 15 open reading frame 58
Calculated Molecular Weight 385 aa, 42 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC127821
Gene Symbol C15orf58
Gene ID (NCBI) 390637
RRIDAB_2880085
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C15orf58 antibody 25447-1-APDownload protocol
IF protocol for C15orf58 antibody 25447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols