• Featured Product
  • KD/KO Validated

CA3 Polyclonal antibody

CA3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15197-1-AP

Synonyms

CA III, CA3, CAIII, Car3, Carbonate dehydratase III, Carbonic anhydrase 3, Carbonic anhydrase III



Tested Applications

Positive WB detected inHEK-293 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, HeLa cells
Positive IHC detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15197-1-AP targets CA3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA3 fusion protein Ag7344
Full Name carbonic anhydrase III, muscle specific
Calculated molecular weight 29 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC004897
Gene symbol CA3
Gene ID (NCBI) 761
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Carbonic anhydrase III (CA3), which belongs to the alpha-carbonic anhydrase family, is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase (PMID: 10064618). CA3 was found to be localized in Type-I muscle fibers and could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases (PMID: 6221502).

Protocols

Product Specific Protocols
WB protocol for CA3 antibody 15197-1-APDownload protocol
IHC protocol for CA3 antibody 15197-1-APDownload protocol
IF protocol for CA3 antibody 15197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

PLoS One

Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation.

Authors - Claes-Göran Reibring
mouseWB

PLoS One

Multifactorial Comparative Proteomic Study of Cytochrome P450 2E1 Function in Chronic Alcohol Administration.

Authors - Yuan Wang
  • KD Validated
ratWB,IHC

J Nutr Biochem

Postnatal high-fat diet sex-specifically exacerbates prenatal dexamethasone-induced hypertension: Mass spectrometry-based quantitative proteomic approach.

Authors - Chien-Ning Hsu