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CD44 Monoclonal antibody

CD44 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

6F4H2

Cat no : 60224-1-Ig

Synonyms

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I



Tested Applications

Positive WB detected inHeLa cells, HUVEC cells, human spleen tissue, pig spleen tissue, A549 cells, MDA-MB-231 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman lung cancer tissue
Positive FC detected inRaji cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60224-1-Ig is CD44 Fusion Protein expressed in E. coli.

Tested Reactivity human, pig
Cited Reactivity human, pig, swine
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD44 fusion protein Ag7633
Full Name CD44 molecule (Indian blood group)
Calculated molecular weight 742 aa, 82 kDa
Observed molecular weight 80-95 kDa
GenBank accession numberBC004372
Gene symbol CD44
Gene ID (NCBI) 960
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.

Protocols

Product Specific Protocols
WB protocol for CD44 antibody 60224-1-IgDownload protocol
IHC protocol for CD44 antibody 60224-1-IgDownload protocol
IF protocol for CD44 antibody 60224-1-IgDownload protocol
FC protocol for CD44 antibody 60224-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

ACS Appl Mater Interfaces

All-Aqueous Phase Microfluidics for Cell Encapsulation.

Authors - Kaixuan Zhu
humanIF

Adv Mater Technol

The Unusual Properties of Polytetrafluoroethylene Enable Massive-Volume Vitrification of Stem Cells with Low-Concentration Cryoprotectants.

Authors - Yuan Cao

Cell Reprogram

Inhibition of DNA Methyltransferase by RG108 Promotes Pluripotency-Related Character of Porcine Bone Marrow Mesenchymal Stem Cells.

Authors - Qi Li
human,pigWB,IF

J Reprod Dev

Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa.

Authors - Álvarez-Rodriguez Manuel
WB

Reprod Domest Anim

Chicken seminal fluid lacks CD9- And CD44-bearing extracellular vesicles.

Authors - Manuel Alvarez-Rodriguez
swineWB

Theriogenology

Exosomes in specific fractions of the boar ejaculate contain CD44: A marker for epididymosomes?

Authors - Manuel Alvarez-Rodriguez