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CD44 Antibody 11 Publications

Mouse Monoclonal| Catalog number: 60224-1-Ig |CloneNo.: 6F4H2

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Con: 191 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.


Images See all

CD44 Antibody WB HeLa cells 60224-1-Ig

WB result of CD44 antibody (60224-1-Ig, 1:4000) with si-Control and si-CD44 transfected HeLa cells.

CD44 Antibody WB HeLa cells 60224-1-Ig

HeLa (CD44+), A549 (CD44+), MDA-MB-231 (CD44+), MCF-7, Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

CD44 Antibody IF human lung cancer tissue 60224-1-Ig

Immunofluorescent analysis of ( 4% PFA) fixed human lung cancer tissue using 60224-1-Ig (CD44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

CD44 Antibody IF human lung cancer tissue 60224-1-Ig

Immunofluorescent analysis of ( 4% PFA) fixed human lung cancer tissue using 60224-1-Ig (CD44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

CD44 Antibody FC Raji cells 60224-1-Ig

1X10^6 Raji cells were stained with 0.2ug CD44 antibody (60224-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-mouse IgG with dilution 1:1000.

CD44 Antibody IHC human lung cancer tissue 60224-1-Ig

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig( CD44 Antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CD44 Antibody IHC human lung cancer tissue 60224-1-Ig

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig( CD44 Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, pig, swine
Positive WB detected in:
HeLa cells, HUVEC cells, human spleen tissue, pig spleen tissue, A549 cells, MDA-MB-231 cells
Positive IHC detected in:
human lung cancer tissue, human breast cancer tissue, human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
human lung cancer tissue
Positive FC detected in:
Raji cells
Recommended dilution:
WB : 1:2000-1:10000
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2a
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD44 fusion protein Ag7633
Full name:
CD44 molecule (Indian blood group)
Calculated molecular weight:
742aa,82 kDa
Observed molecular weight:
80-95 kDa
GenBank accession number:
BC004372
Gene ID (NCBI):
960
Gene symbol
CD44
Synonyms
CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

Background

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.


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