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  • KD/KO Validated

CDC6 Polyclonal antibody

CDC6 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11640-1-AP

Synonyms

Cdc18 related protein, CDC18L, CDC6, CDC6 related protein, HsCDC18, HsCDC6, p62(cdc6)



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, SMMC-7721 cells
Positive IHC detected inmouse brain tissue, human colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11640-1-AP targets CDC6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC6 fusion protein Ag2228
Full Name cell division cycle 6 homolog (S. cerevisiae)
Calculated molecular weight 560 aa, 63 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC025232
Gene symbol CDC6
Gene ID (NCBI) 990
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. The active Cdc6 functioning as a dimer at replication origins (10793143,15096526). It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CDC6.

Protocols

Product Specific Protocols
WB protocol for CDC6 antibody 11640-1-APDownload protocol
IHC protocol for CDC6 antibody 11640-1-APDownload protocol
IF protocol for CDC6 antibody 11640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Genes Cells

YB-1 promotes transcription of cyclin D1 in human non-small-cell lung cancers.

Authors - Masanori Harada
humanWB

Onco Targets Ther

Circular RNA circNELL2 Acts as the Sponge of miR-127-5p to Promote Esophageal Squamous Cell Carcinoma Progression.

Authors - Gang Xiong
humanWB

Cancer Lett

N-myc downstream regulated gene1/Cap43 overexpression suppresses tumor growth by hepatic cancer cells through cell cycle arrest at the G0/G1 phase.

Authors - Akiba Jun J
humanWB

Oncotarget

PRMT5 competitively binds to CDK4 to promote G1-S transition upon glucose induction in hepatocellular carcinoma.

Authors - Hao Yang
humanWB

Oncotarget

The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.

Authors - Lifei Fan
humanWB,IHC

Eur J Cancer

Y-box binding protein-1 (YB-1) promotes cell cycle progression through CDC6-dependent pathway in human cancer cells.

Authors - Basaki Yuji Y
  • KD Validated