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CDC6 Polyclonal antibody

CDC6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11640-1-AP

Synonyms

Cdc18 related protein, CDC18L, CDC6, CDC6 related protein, HsCDC18, HsCDC6, p62(cdc6)



Tested Applications

Positive WB detected inJurkat cells, SMMC-7721 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue, human colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11640-1-AP targets CDC6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC6 fusion protein Ag2228
Full Name cell division cycle 6 homolog (S. cerevisiae)
Calculated Molecular Weight 560 aa, 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC025232
Gene Symbol CDC6
Gene ID (NCBI) 990
RRIDAB_2078519
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. The active Cdc6 functioning as a dimer at replication origins (10793143,15096526). It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CDC6.

Protocols

Product Specific Protocols
WB protocol for CDC6 antibody 11640-1-APDownload protocol
IHC protocol for CDC6 antibody 11640-1-APDownload protocol
IF protocol for CDC6 antibody 11640-1-APDownload protocol
IP protocol for CDC6 antibody 11640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cancer

A novel LncRNA transcript, RBAT1, accelerates tumorigenesis through interacting with HNRNPL and cis-activating E2F3.

Authors - Xiaoyu He
humanWB

Adv Sci (Weinh)

Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer

Authors - Rong Wang
humanIF

Proc Natl Acad Sci U S A

Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase.

Authors - Yim Hyungshin H
mouseIF

PLoS Genet

Histone deacetylase 2 (HDAC2) regulates chromosome segregation and kinetochore function via H4K16 deacetylation during oocyte maturation in mouse.

Authors - Ma Pengpeng P
humanWB

Int J Mol Sci

Norcantharidin Induces Immunogenic Cell Death of Bladder Cancer Cells through Promoting Autophagy in Acidic Culture.

Authors - Lili Xu
humanWB

Aging (Albany NY)

β-Elemene enhances radiosensitivity in non-small-cell lung cancer by inhibiting epithelial-mesenchymal transition and cancer stem cell traits via Prx-1/NF-kB/iNOS signaling pathway.

Authors - Kun Zou