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CDK5 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 10430-1-AP

Featured Product KD/KO validated

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IF, ELISA
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CDK5 Antibody WB NIH/3T3 cells 10430-1-AP

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10430-1-AP(CDK5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

CDK5 Antibody IF NIH/3T3 cells 10430-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed NIH/3T3 cells using 10430-1-AP(CDK5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
NIH/3T3 cells, mouse pancreas tissue
Positive IF detected in:
NIH/3T3 cells
Recommended dilution:
WB : 1:500-1:1000
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CDK5 fusion protein Ag0686
Full name:
cyclin-dependent kinase 5
Calculated molecular weight:
31 kDa or 33 kDa
Observed molecular weight:
33 kDa
GenBank accession number:
BC005115
Gene ID (NCBI):
1020
Gene symbol
CDK5
Synonyms
CDK5, Cell division protein kinase 5, cyclin dependent kinase 5, PSSALRE, TPKII catalytic subunit

Background

Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.


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