Filter: AllWBIPIHCIF/ICC

WB analysis using 10430-1-AP
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WB analysis using 10430-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10430-1-AP (CDK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10430-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10430-1-AP (CDK5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 10430-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10430-1-AP (CDK5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10430-1-AP
IP result of anti-CDK5 (IP:10430-1-AP, 4ug; Detection:10430-1-AP 1:3000) with mouse brain tissue lysate 2120 ug.
IHC staining of mouse kidney using 10430-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10430-1-AP (CDK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 10430-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10430-1-AP (CDK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 10430-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10430-1-AP (CDK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 10430-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10430-1-AP (CDK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 10430-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10430-1-AP (CDK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 10430-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10430-1-AP (CDK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of SH-SY5Y using 10430-1-AP
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CDK5 antibody (10430-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

CDK5 Polyclonal antibody

CDK5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10430-1-AP

Publications(18)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Cdk 5, Cyclin-dependent kinase 5, Cyclin-dependent-like kinase 5, EC:2.7.11.1, PSSALRE

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (18)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, HCT 116 cells, mouse pancreas tissue, NIH/3T3 cells, WB result of CDK5 antibody (10430-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and CDK5 knockout HCT 116 cells., mouse brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse kidney tissue, human renal cell carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 16 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

10430-1-AP targets CDK5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CDK5 fusion protein Ag0686 Predict reactive species
Full Name	cyclin-dependent kinase 5
Calculated Molecular Weight	31 kDa, 33 kDa
Observed Molecular Weight	33 kDa
GenBank Accession Number	BC005115
Gene Symbol	CDK5
Gene ID (NCBI)	1020
RRID	AB_2078859
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q00535
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.

Protocols

Product Specific Protocols
WB protocol for CDK5 antibody 10430-1-AP	Download protocol
IHC protocol for CDK5 antibody 10430-1-AP	Download protocol
IF protocol for CDK5 antibody 10430-1-AP	Download protocol
IP protocol for CDK5 antibody 10430-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis Authors - Saisai Guo View Article
mouse	WB	Neuropharmacology Inhibition of Cdk5 rejuvenates inhibitory circuits and restores experience-dependent plasticity in adult visual cortex. Authors - Yue Li KD Validated View Article
human	WB	Cells Phosphorylation-Induced Ubiquitination and Degradation of PXR through CDK2-TRIM21 Axis. Authors - Mengyao Qin KD Validated View Article
human	WB	Am J Transl Res Hypoxia induces HT-22 neuronal cell death via Orai1/CDK5 pathway-mediated Tau hyperphosphorylation. Authors - Binbin Fang View Article
mouse	WB	J Cell Biochem Nestin protects mouse podocytes against high glucose-induced apoptosis by a Cdk5-dependent mechanism. Authors - Liu Wei W View Article
mouse,human	WB	BMC Neurol The LPA-CDK5-tau pathway mediates neuronal injury in an in vitro model of ischemia-reperfusion insult. Authors - Yaya Wang View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH XinN (Verified Customer) (02-27-2023)	It can recognize endogenous CDK5 with a right MW. Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: mouse brain lysate CDK5 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:10430-1-AP)
FH Kathryn (Verified Customer) (04-22-2022)	This CDK5 antibody works very well to stain the nuclei of developed mouse oocytes. Applications: Immunofluorescence Primary Antibody Dilution: 1:100 Cell Tissue Type: Mouse ovary sections CDK5 Antibody Immunofluorescence validation (1:100 dilution) in Mouse ovary sections (Cat no:10430-1-AP)