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- KD/KO Validated
CDK5 Polyclonal antibody
CDK5 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10430-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, mouse pancreas tissue, NIH/3T3 cells, mouse brain tissue |
Positive IHC detected in | mouse kidney tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | NIH/3T3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 5 publications below |
Product Information
10430-1-AP targets CDK5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CDK5 fusion protein Ag0686 |
Full Name | cyclin-dependent kinase 5 |
Calculated molecular weight | 31 kDa, 33 kDa |
Observed molecular weight | 33 kDa |
GenBank accession number | BC005115 |
Gene symbol | CDK5 |
Gene ID (NCBI) | 1020 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.
Protocols
Product Specific Protocols | |
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WB protocol for CDK5 antibody 10430-1-AP | Download protocol |
IHC protocol for CDK5 antibody 10430-1-AP | Download protocol |
IF protocol for CDK5 antibody 10430-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Br J Pharmacol Potent effects of dioscin against hepatocellular carcinoma through regulating TIGAR-mediated apoptosis, autophagy and DNA damage. | ||
Mol Biol Rep Ginsenoside compound K ameliorates Alzheimer's disease in HT22 cells by adjusting energy metabolism. | ||
J Cell Biochem Nestin protects mouse podocytes against high glucose-induced apoptosis by a Cdk5-dependent mechanism. | ||
Am J Transl Res Hypoxia induces HT-22 neuronal cell death via Orai1/CDK5 pathway-mediated Tau hyperphosphorylation. | ||
Neuropharmacology Inhibition of Cdk5 rejuvenates inhibitory circuits and restores experience-dependent plasticity in adult visual cortex.
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