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CDK5 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 10430-1-AP

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

CDK5 Antibody WB HepG2 cells 10430-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10430-1-AP (CDK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

CDK5 Antibody IHC mouse kidney tissue 10430-1-AP

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10430-1-AP (CDK5 antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CDK5 Antibody IHC mouse kidney tissue 10430-1-AP

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10430-1-AP (CDK5 antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CDK5 Antibody IF NIH/3T3 cells 10430-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed NIH/3T3 cells using 10430-1-AP(CDK5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HepG2 cells, mouse pancreas tissue, NIH/3T3 cells, mouse brain tissue
Positive IHC detected in:
mouse kidney tissue, human renal cell carcinoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
NIH/3T3 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CDK5 fusion protein Ag0686
Full name:
cyclin-dependent kinase 5
Calculated molecular weight:
31 kDa or 33 kDa
Observed molecular weight:
33 kDa
GenBank accession number:
BC005115
Gene ID (NCBI):
1020
Gene symbol
CDK5
Synonyms
CDK5, Cell division protein kinase 5, cyclin dependent kinase 5, PSSALRE, TPKII catalytic subunit

Background

Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.


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