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CDK5 Polyclonal antibody

CDK5 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10430-1-AP

Synonyms

CDK5, Cell division protein kinase 5, cyclin dependent kinase 5, PSSALRE, TPKII catalytic subunit



Tested Applications

Positive WB detected inHepG2 cells, mouse pancreas tissue, NIH/3T3 cells, mouse brain tissue
Positive IHC detected inmouse kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10430-1-AP targets CDK5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK5 fusion protein Ag0686
Full Name cyclin-dependent kinase 5
Calculated molecular weight 31 kDa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC005115
Gene symbol CDK5
Gene ID (NCBI) 1020
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.

Protocols

Product Specific Protocols
WB protocol for CDK5 antibody 10430-1-APDownload protocol
IHC protocol for CDK5 antibody 10430-1-APDownload protocol
IF protocol for CDK5 antibody 10430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Br J Pharmacol

Potent effects of dioscin against hepatocellular carcinoma through regulating TIGAR-mediated apoptosis, autophagy and DNA damage.

Authors - Zhang Mao
mouseWB

Mol Biol Rep

Ginsenoside compound K ameliorates Alzheimer's disease in HT22 cells by adjusting energy metabolism.

Authors - Xijun Chen
mouseWB

J Cell Biochem

Nestin protects mouse podocytes against high glucose-induced apoptosis by a Cdk5-dependent mechanism.

Authors - Liu Wei W
humanWB

Am J Transl Res

Hypoxia induces HT-22 neuronal cell death via Orai1/CDK5 pathway-mediated Tau hyperphosphorylation.

Authors - Binbin Fang
mouseWB

Neuropharmacology

Inhibition of Cdk5 rejuvenates inhibitory circuits and restores experience-dependent plasticity in adult visual cortex.

Authors - Yue Li
  • KD Validated