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  • Featured Product
  • KD/KO Validated

CHCHD4 Polyclonal antibody

CHCHD4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 21090-1-AP

Synonyms

CHCHD4, MIA40

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Daudi cells using CHCHD4 Polyclonal antibody (21090-1-AP)

    WB analysis of Daudi using 21090-1-AP

    Daudi cells were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using CHCHD4 Polyclonal antibody (21090-1-AP)

    WB analysis of Raji using 21090-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using CHCHD4 Polyclonal antibody (21090-1-AP)

    WB analysis of rat brain using 21090-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CHCHD4 Polyclonal antibody (21090-1-AP)

    WB analysis of mouse brain using 21090-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using CHCHD4 Polyclonal antibody (21090-1-AP)

    WB analysis of human heart using 21090-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CHCHD4 Polyclonal antibody (21090-1-AP)

    WB analysis of mouse lung using 21090-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using CHCHD4 Polyclonal antibody (21090-1-AP)

    WB analysis of mouse spleen using 21090-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using CHCHD4 Polyclonal antibody (21090-1-AP)

    WB analysis of L02 using 21090-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ramos cells using CHCHD4 Polyclonal antibody (21090-1-AP)

    WB analysis of Ramos using 21090-1-AP

    Ramos cells were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using CHCHD4 Polyclonal antibody (21090-1-AP)

    WB analysis of mouse kidney using 21090-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CHCHD4 Polyclonal antibody (21090-1-AP)

    IP experiment of mouse brain using 21090-1-AP

    IP result of anti-CHCHD4 (IP:21090-1-AP, 4ug; Detection:21090-1-AP 1:1000) with mouse brain tissue lysate 3200 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CHCHD4 Polyclonal antibody (21090-1-AP)

    IHC staining of mouse testis using 21090-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21090-1-AP (CHCHD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CHCHD4 Polyclonal antibody (21090-1-AP)

    IHC staining of mouse testis using 21090-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21090-1-AP (CHCHD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CHCHD4 Polyclonal antibody (21090-1-AP)

    IHC staining of human kidney using 21090-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21090-1-AP (CHCHD4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CHCHD4 Polyclonal antibody (21090-1-AP)

    IHC staining of human kidney using 21090-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21090-1-AP (CHCHD4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CHCHD4 Polyclonal antibody (21090-1-AP)

    IF Staining of A431 using 21090-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CHCHD4 antibody (21090-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inDaudi cells, human heart tissue, mouse lung tissue, mouse spleen tissue, L02 cells, mouse kidney tissue, Ramos cells, Raji cells, rat brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21090-1-AP targets CHCHD4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHCHD4 fusion protein Ag15339
Full Name coiled-coil-helix-coiled-coil-helix domain containing 4
Calculated Molecular Weight 142 aa, 16 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC033775
Gene Symbol CHCHD4
Gene ID (NCBI) 131474
RRIDAB_10734583
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHCHD4, a component of human mitochondria, belongs to a protein family. It functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. It is required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS).

Protocols

Product Specific Protocols
WB protocol for CHCHD4 antibody 21090-1-APDownload protocol
IHC protocol for CHCHD4 antibody 21090-1-APDownload protocol
IF protocol for CHCHD4 antibody 21090-1-APDownload protocol
IP protocol for CHCHD4 antibody 21090-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Stem Cell

Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.

Authors - Rashim Pal Singh
View Article
ratWB,IF

Exp Mol Med

SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis

Authors - Jianxin Mao
View Article
humanWB,IF

Aging Cell

Dependence of PINK1 accumulation on mitochondrial redox system.

Authors - Feng Gao
  • KD Validated
View Article
humanWB

Redox Biol

Flow-induced endothelial mitochondrial remodeling mitigates mitochondrial reactive oxygen species production and promotes mitochondrial DNA integrity in a p53-dependent manner.

Authors - Junchul Shin
View Article
humanWB

EMBO Mol Med

A novel CHCHD10 mutation implicates a Mia40-dependent mitochondrial import deficit in ALS.

Authors - Carina Lehmer
View Article
humanWB

Oncogene

CHTM1, a novel metabolic marker deregulated in human malignancies.

Authors - Mansi Babbar
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Veronica (Verified Customer) (10-17-2023)

First time I find an anti-CHCHD4 antibody that works fine. We are testing it in IF as well, but till now I am already really happy with the result in WB. No aspecific signal, clear band. Reccomended! Conditions: Gradient gel 8-15% Ab’: 1:1000 in 5% milk O/N

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T; HeLa; isolated mitochondria from HEK293T
CHCHD4 Antibody Western Blot validation (1:1000 dilution) in HEK293T; HeLa; isolated mitochondria from HEK293T (Cat no:21090-1-AP)
FH

Süleyman (Verified Customer) (01-23-2022)

CHCHD4 antibody (Green) is used 1:100 dilution (2 hour at RT) and 1:700 of Alexa Fluor 488 secondary antibody (1 hour RT). 4% Paraformaldehyde fixation for 10 min at RT. Permeabilization with 0.3% Triton X for 10-15 minutes at RT. 5% FBS in PBS used for blocking for 1 hour at RT. The image is colocalized with TOMM20 (Red). Blue is DAPI. As you can see there is colocalization with TOMM20 as CHCHD4 supposed to be in IMS but I also see unspecific bindings in cytosolic proteins. Therefore my rating is 3 as it might be binding unspecifically.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: HeLa FlipIn
CHCHD4 Antibody Immunofluorescence validation (1:100 dilution) in HeLa FlipIn (Cat no:21090-1-AP)
FH

Lana (Verified Customer) (08-13-2019)

SDS-PAGE: 10 ug/ul RIPA lysate of the mitochondrial fraction of HEK293t. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4CSecondary Ab: IRDye 800CW Goat anti-Rabbit

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293t
CHCHD4 Antibody Western Blot, validation (1:1000 dilution) in HEK293t (Cat no:21090-1-AP)