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CLINT1 Polyclonal antibody

CLINT1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10470-1-AP

Synonyms

clathrin interactor 1, CLINT, CLINT1, ENTH, Enthoprotin, EPN4, EPNR, Epsin 4, Epsin related protein, EPSINR, KIAA0171

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CLINT1 Polyclonal antibody (10470-1-AP)

    WB analysis of HeLa using 10470-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10470-1-AP (CLINT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CLINT1 Polyclonal antibody (10470-1-AP)

    WB analysis of HEK-293 using 10470-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10470-1-AP (CLINT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CLINT1 Polyclonal antibody (10470-1-AP)

    WB analysis of mouse brain using 10470-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10470-1-AP (CLINT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CLINT1 Polyclonal antibody (10470-1-AP)

    WB analysis of HepG2 using 10470-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10470-1-AP (CLINT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CLINT1 Polyclonal antibody (10470-1-AP)

    IP experiment of HeLa using 10470-1-AP

    IP result of anti-CLINT1 (IP:10470-1-AP, 4ug; Detection:10470-1-AP 1:1000) with HeLa cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CLINT1 Polyclonal antibody (10470-1-AP)

    IHC staining of human breast cancer using 10470-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10470-1-AP (CLINT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CLINT1 Polyclonal antibody (10470-1-AP)

    IHC staining of human breast cancer using 10470-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10470-1-AP (CLINT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CLINT1 Polyclonal antibody (10470-1-AP)

    IF Staining of HepG2 using 10470-1-AP

    Immunofluorescent analysis of HepG2 cells, using CLINT1antibody 10470-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using CLINT1 Polyclonal antibody (10470-1-AP)

    FC experiment of HepG2 using 10470-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug CLINT1 antibody (10470-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10470-1-AP targets CLINT1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLINT1 fusion protein Ag0754
Full Name clathrin interactor 1
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa, 80 kDa
GenBank Accession NumberBC004467
Gene Symbol CLINT1
Gene ID (NCBI) 9685
RRIDAB_2276405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Clathrin interactor 1 (CLINT1), also known as enthoprotin or epsin-4, belongs to the epsin family of endocytic adapter proteins. CLINT1 interacts with clathrin, the adapter protein AP-1 and phosphoinositides. It may have a role in the formation of clathrin coated vesicles and trafficking between the trans-Golgi network and endosomes. Mutations in the gene of CLINT1 are associated with a susceptibility to schizophrenia and psychotic disorders. Calculated molecular weight of CLINT1 is 68 kDa. The migration of endogenous CLINT1 at 80 kDa has also been reported (PMID: 12213833; 15811338).

Protocols

Product Specific Protocols
WB protocol for CLINT1 antibody 10470-1-APDownload protocol
IHC protocol for CLINT1 antibody 10470-1-APDownload protocol
IF protocol for CLINT1 antibody 10470-1-APDownload protocol
IP protocol for CLINT1 antibody 10470-1-APDownload protocol
FC protocol for CLINT1 antibody 10470-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

BioE3 identifies specific substrates of ubiquitin E3 ligases

Authors - Orhi Barroso-Gomila
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Joleen (Verified Customer) (06-16-2019)

Recognizes CLINT1 prominently with some background, non-specific bands that were of lower intensity than CLINT1.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MDCK GII
CLINT1 Antibody Western Blot, validation (1:1000 dilution) in MDCK GII (Cat no:10470-1-AP)