Validation Data Gallery
|Positive WB detected in||human plasma tissue|
|Positive IHC detected in||human tonsillitis tissue, human breast cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12289-1-AP targets Clusterin in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Clusterin fusion protein Ag2889|
|Calculated molecular weight||449 aa, 53 kDa|
|Observed molecular weight||37 kDa|
|GenBank accession number||BC010514|
|Gene ID (NCBI)||1191|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).
Proc Natl Acad Sci U S A
Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.
Platelets contribute to amyloid-β aggregation in cerebral vessels through integrin αIIbβ3-induced outside-in signaling and clusterin release.
Front Cell Dev Biol
Structural and Functional Characterization of Fibronectin in Extracellular Vesicles From Hepatocytes.
Ecotoxicol Environ Saf
Identification and functional analysis of senescence-associated secretory phenotype of premature senescent hepatocytes induced by hexavalent chromium.
Food Chem Toxicol
Clusterin overexpression as a potential neuroprotective response to the pathological effects of high fat dieting on the brain reward system.
Ecotoxicol Environ Saf
Expression of Clusterin suppresses Cr(VI)-induced premature senescence through activation of PI3K/AKT pathway.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
George (Verified Customer) (12-03-2018)
IHC performed on paraffin-embedded human kidney tissue