Clusterin Polyclonal antibody

Clusterin Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12289-1-AP

Synonyms

AAG4, Apo J, APOJ, ApoJalpha, ApoJbeta, Apolipoprotein J, CLI, CLU, clusterin, Complement cytolysis inhibitor, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, TRPM 2, TRPM2



Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12289-1-AP targets Clusterin in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Clusterin fusion protein Ag2889
Full Name clusterin
Calculated molecular weight 449 aa, 53 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC010514
Gene symbol CLU
Gene ID (NCBI) 1191
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

Protocols

Product Specific Protocols
WB protocol for Clusterin antibody 12289-1-APDownload protocol
IHC protocol for Clusterin antibody 12289-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.

Authors - Lei Shi

Sci Signal

Platelets contribute to amyloid-β aggregation in cerebral vessels through integrin αIIbβ3-induced outside-in signaling and clusterin release.

Authors - Lili Donner
mouseWB

Front Cell Dev Biol

Structural and Functional Characterization of Fibronectin in Extracellular Vesicles From Hepatocytes.

Authors - Xinlei Li
humanWB

Ecotoxicol Environ Saf

Identification and functional analysis of senescence-associated secretory phenotype of premature senescent hepatocytes induced by hexavalent chromium.

Authors - Yu Ma
mouseIHC

Food Chem Toxicol

Clusterin overexpression as a potential neuroprotective response to the pathological effects of high fat dieting on the brain reward system.

Authors - Carmen Rodríguez-Rivera
humanCoIP

Ecotoxicol Environ Saf

Expression of Clusterin suppresses Cr(VI)-induced premature senescence through activation of PI3K/AKT pathway.

Authors - Yujing Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

George (Verified Customer) (12-03-2018)

IHC performed on paraffin-embedded human kidney tissue

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: human
Clusterin Antibody Immunohistochemistry, validation (1:200 dilution) in human (Cat no:12289-1-AP)