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  • KD/KO Validated

CNPY2,MSAP Polyclonal antibody

CNPY2,MSAP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14635-1-AP

Synonyms

canopy 2 homolog (zebrafish), CNPY2, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9



Tested Applications

Positive WB detected inmouse pancreas tissue, human liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14635-1-AP targets CNPY2,MSAP in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNPY2,MSAP fusion protein Ag6214
Full Name canopy 2 homolog (zebrafish)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC065015
Gene symbol CNPY2
Gene ID (NCBI) 10330
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2

Protocols

Product Specific Protocols
WB protocol for CNPY2,MSAP antibody 14635-1-APDownload protocol
IHC protocol for CNPY2,MSAP antibody 14635-1-APDownload protocol
IF protocol for CNPY2,MSAP antibody 14635-1-APDownload protocol
IP protocol for CNPY2,MSAP antibody 14635-1-APDownload protocol
FC protocol for CNPY2,MSAP antibody 14635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Int J Mol Med

Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway.

Authors - Yue Cui
ratWB

J Cell Mol Med

PMID: 31625681

Authors - Lina Zhao
humanWB

Front Pharmacol

PMID: 31396081

Authors - Chong Liu
mouseWB

J Biol Chem

Fibroblast growth factor-21 (FGF21) regulates low-density lipoprotein receptor (LDLR) levels in cells via the E3-ubiquitin ligase Mylip/Idol and the Canopy2 (Cnpy2)/Mylip-interacting saposin-like protein (Msap).

Authors - Do Hai Thi HT
  • KD Validated
ratWB

J Biol Chem

Reciprocal regulation of Very low-density lipoprotein receptors (VLDLRs) in neurons by Brain-derived neurotrophic factor (BDNF) and Reelin: Involvement of the E3 ligase Mylip/Idol.

Authors - Do Hai Thi HT
human,mouseWB

Nat Struct Mol Biol

CNPY2 is a key initiator of the PERK-CHOP pathway of the unfolded protein response.

Authors - Feng Hong
  • KO Validated