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Collagen Type VI Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 14853-1-AP

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Con: 20 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

Collagen Type VI Antibody WB human heart tissue 14853-1-AP

human heart tissue were subjected to SDS PAGE followed by western blot with 14853-1-AP(Collagen Type VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Collagen Type VI Antibody IHC human heart tissue 14853-1-AP

Immunohistochemical of paraffin-embedded human heart using 14853-1-AP(Collagen Type VI antibody) at dilution of 1:50 (under 40x lens)

Collagen Type VI Antibody IHC human heart tissue 14853-1-AP

Immunohistochemical of paraffin-embedded human heart using 14853-1-AP(Collagen Type VI antibody) at dilution of 1:50 (under 10x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
human heart tissue
Positive IHC detected in:
human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Collagen Type VI fusion protein Ag6635
Full name:
collagen, type VI, alpha 2
Calculated molecular weight:
109 kDa
Observed molecular weight:
140 kDa
GenBank accession number:
BC065509
Gene ID (NCBI):
1292
Gene symbol
COL6A2
Synonyms
COL6A2, Collagen alpha 2(VI) chain, Collagen Type VI, collagen, type VI, alpha 2, PP3610

Background

Collagen VI, composed of three polypeptides, alpha-1(VI), alpha-2(VI), and alpha-3(VI) chains, is a beaded filament collagen found in most connective tissues. The alpha-1(VI) and alpha-2(VI) chains are 140 kDa, while the alpha-3(VI) chain is 200-300 kDa (PMID: 3792302, 3348212). COL6A2 gene encodes the alpha-2 subunit of type VI collagen. The alpha-2(VI) subunit has a calculated molecular weight of 109 kDa, and an apparent molecular weight of 140 kDa due to posttranslational modification. Mutations in COL6A2 gene are associated with Bethlem myopathy and Ullrich scleroatonic muscular dystrophy. Three transcript variants have been identified for this gene.


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