CSTF2T Polyclonal antibody

CSTF2T Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14206-1-AP

Synonyms

CstF 64T, CSTF2T, KIAA0689, TauCstF 64



Tested Applications

Positive WB detected inA549 cells, rat brain tissue, human brain tissue, HeLa cells, HepG2 cells, mouse liver tissue
Positive IP detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14206-1-AP targets CSTF2T in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSTF2T fusion protein Ag5411
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, tau variant
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC028239
Gene Symbol CSTF2T
Gene ID (NCBI) 23283
RRIDAB_2086478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CSTF2T antibody 14206-1-APDownload protocol
IHC protocol for CSTF2T antibody 14206-1-APDownload protocol
IF protocol for CSTF2T antibody 14206-1-APDownload protocol
IP protocol for CSTF2T antibody 14206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols