A375 cells were subjected to SDS PAGE followed by western blot with 12216-1-AP(CTSB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

HT-1080 cells were subjected to SDS PAGE followed by western blot with 12216-1-AP(CTSB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

human brain tissue were subjected to SDS PAGE followed by western blot with 12216-1-AP(CTSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

human liver tissue were subjected to SDS PAGE followed by western blot with 12216-1-AP(CTSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 12216-1-AP(CTSB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 12216-1-AP(CTSB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 12216-1-AP(CTSB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12216-1-AP(CTSB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

RAW264.7 cells were subjected to SDS PAGE followed by western blot with 12216-1-AP(CTSB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

RAW264.7 cells were subjected to SDS PAGE followed by western blot with 12216-1-AP(CTSB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

Immunohistochemical of paraffin-embedded human breast cancer using 12216-1-AP(CTSB antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human breast cancer using 12216-1-AP(CTSB antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical of paraffin-embedded human colon cancer using 12216-1-AP(CTSB antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human colon cancer using 12216-1-AP(CTSB antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 12216-1-AP( Cathepsin B Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 12216-1-AP( Cathepsin B Antibody) at dilution of 1:200 (under 40x lens).

Immunofluorescent analysis of A375 cells using 12216-1-AP(Cathepsin B antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

1X10^6 MCF-7 cells were stained with 0.2ug Cathepsin B antibody (12216-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.