Cathepsin B Polyclonal antibody
Cathepsin B Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 12216-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, mouse brain tissue, NIH/3T3 cells, HUVEC cells |
| Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A375 cells |
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 11 publications below |
| IHC | See 2 publications below |
Product Information
12216-1-AP targets Cathepsin B in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | frog, human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cathepsin B fusion protein Ag2898 |
| Full Name | cathepsin B |
| Calculated molecular weight | 339 aa, 38 kDa |
| Observed molecular weight | 38 kDa, 44 kDa |
| GenBank accession number | BC010240 |
| Gene symbol | CTSB |
| Gene ID (NCBI) | 1508 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 43 kDa form of pro-CTSB, 31 kDa and 25 kDa marure forms in mouse brain by western blot(PMID:20616152).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cathepsin B antibody 12216-1-AP | Download protocol |
| IHC protocol for Cathepsin B antibody 12216-1-AP | Download protocol |
| IF protocol for Cathepsin B antibody 12216-1-AP | Download protocol |
| FC protocol for Cathepsin B antibody 12216-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncotarget Adipocyte-derived exosomes promote lung cancer metastasis by increasing MMP9 activity via transferring MMP3 to lung cancer cells. | ||
Cell Death Dis A novel acridine derivative, LS-1-10 inhibits autophagic degradation and triggers apoptosis in colon cancer cells. | ||
Toxicol Appl Pharmacol Alantolactone induces apoptosis and improves chemosensitivity of pancreatic cancer cells by impairment of autophagy-lysosome pathway via targeting TFEB. | ||
Oncol Lett Expression profile of cathepsins indicates the potential of cathepsins B and D as prognostic factors in breast cancer patients. | ||
Int J Clin Exp Pathol Expression of cystatin C and its effect on EC9706 cells in esophageal carcinoma. |
