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Cathepsin G Polyclonal antibody

Cathepsin G Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 23840-1-AP

Synonyms

Cathepsin G, CG, CTSG

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using Cathepsin G Polyclonal antibody (23840-1-AP)

    WB analysis of MCF-7 using 23840-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 23840-1-AP (Cathepsin G antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using Cathepsin G Polyclonal antibody (23840-1-AP)

    WB analysis of MDA-MB-453s using 23840-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 23840-1-AP (Cathepsin G antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF-7 cells, MDA-MB-453s cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 23840-1-AP is Cathepsin G Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cathepsin G fusion protein Ag20818
Full Name cathepsin G
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa, 35 kDa
GenBank Accession NumberBC014460
Gene Symbol CTSG
Gene ID (NCBI) 1511
RRIDAB_2879335
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSG(Cathepsin G) is also named as CTLA, CG, MGC23078, Catg and belongs to the peptidase S1 family. It may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. CTSG may also play a role in the progression to heart failure by activating angiotensin II, leading to detrimental effects on the heart. The full length protein contains 255 amino acid and has a signal peptide, a propeptide and a glycosylation site.

Protocols

Product Specific Protocols
WB protocol for Cathepsin G antibody 23840-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation.

Authors - Yansen Xiao
View Article
humanWB

Front Immunol

A Novel Immune-Related Gene Signature to Identify the Tumor Microenvironment and Prognose Disease Among Patients With Oral Squamous Cell Carcinoma Patients Using ssGSEA: A Bioinformatics and Biological Validation Study

Authors - Yun Chen
View Article

PLoS One

Stable depletion of RUNX1-ETO in Kasumi-1 cells induces expression and enhanced proteolytic activity of Cathepsin G and Neutrophil Elastase.

Authors - Caroline Schoenherr
View Article