A549 cells were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Various lysates were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

HT-1080 cells were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

MCF-7 cells were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IP Result of anti-Cortactin (IP:11381-1-AP, 4ug; Detection:11381-1-AP 1:300) with HEK-293 cells lysate 2000ug.

Immunofluorescent analysis of HeLa cells using 11381-1-AP(Cortactin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

1X10^6 HeLa cells were stained with 0.2ug Cortactin antibody (11381-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.