Tested Applications
| Positive WB detected in | Control |
| Positive IP detected in | Control |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30000-0-AP targets Rabbit IgG control in WB, IHC, IP, CoIP, ChIP, RIP, IP-MS, ELISA, Blocking assay, Cell treatment, CUT&RUN applications and shows reactivity with n/a samples.
| Tested Reactivity | n/a |
| Cited Reactivity | hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | Rabbit IgG control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2819035 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Publications
What published studies show
The Rabbit IgG control Polyclonal antibody (catalog 30000-0-AP) has 1,159 total citations. It appears in high-impact journals including Nature, Cell, Cancer Cell, Signal Transduction and Targeted Therapy, Nature Communications, Nature Cell Biology, Molecular Cell, Autophagy, and Nucleic Acids Research. Research fields span cancer biology, autophagy, ferroptosis, epigenetics, immunology, metabolism, and neuroscience, where it serves as an isotype control in IP, CoIP, ChIP, RIP, and WB experiments.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Krüppel-like factor 4 downregulation during esophageal cancer formation facilitates immune evasion and impairs immunotherapy | ||
Signal Transduct Target Ther Glibenclamide targets MDH2 to relieve aging phenotypes through metabolism-regulated epigenetic modification | ||
Cancer Cell Epithelial cells activate fibroblasts to promote esophageal cancer development | ||
Cancer Cell Interferon-dependent SLC14A1(+) cancer-associated fibroblasts promote cancer stemness via WNT5A in bladder cancer | ||
Cancer Cell Targeting tumor monocyte-intrinsic PD-L1 by rewiring STING signaling and enhancing STING agonist therapy |
Reviews
What customers say
Across 10 reviews, 30000-0-AP earns an average rating of 4.7/5. Users primarily employ it as a negative control for immunoprecipitation, consistently reporting clean, reliable results in Jurkat cells, glioblastoma lines, and C. elegans. It also performs well in Western Blot (effective at 1:10,000 dilution) and as a control in neutralization experiments. One reviewer noted the antibody is highly concentrated, recommending using less than typical. Overall, customers describe it as an efficient, dependable isotype control with excellent specificity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
S (Verified Customer) (11-20-2025) |
|
Roy (Verified Customer) (10-07-2025) | Perfect control for IP
|
Celine (Verified Customer) (07-09-2025) | Works well as a negative control for immunoprecipitation
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Siddharth (Verified Customer) (08-02-2023) | Very efficient secondary antibody. Can be used effectively at 1:10,000 dilution.
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Macarena (Verified Customer) (10-07-2022) | very concentrated. Excellent results. Deffinetly use less than you would normally use.
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Guo-rong (Verified Customer) (08-30-2022) | It works well as an antibody control in neutralization experiments
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Anita (Verified Customer) (01-04-2022) | Works well as a control for IP
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Kenneth (Verified Customer) (02-14-2019) | Great company with Great products. Highly recommended.
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Priyanka (Verified Customer) (12-18-2018) |
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Seongjin (Verified Customer) (12-14-2018) |
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