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  • Featured Product
  • KD/KO Validated

CUGBP1 Polyclonal antibody

CUGBP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13002-1-AP

Synonyms

Bruno like protein 2, BRUNOL2, CELF 1, CELF1, CUG BP, CUG BP1, CUGBP, CUGBP1, Deadenylation factor CUG BP, EDEN BP homolog, hNab50, NAB50, RNA binding protein BRUNOL 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CUGBP1 Polyclonal antibody (13002-1-AP)

    WB analysis using 13002-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13002-1-AP (CUGBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CUGBP1 Polyclonal antibody (13002-1-AP)

    WB analysis of HeLa using 13002-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13002-1-AP (CUGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using CUGBP1 Polyclonal antibody (13002-1-AP)

    WB analysis of NIH/3T3 using 13002-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13002-1-AP (CUGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using CUGBP1 Polyclonal antibody (13002-1-AP)

    WB analysis of HT-1080 using 13002-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 13002-1-AP (CUGBP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using CUGBP1 Polyclonal antibody (13002-1-AP)

    WB analysis of HL-60 using 13002-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13002-1-AP (CUGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CUGBP1 Polyclonal antibody (13002-1-AP)

    IP experiment of HeLa using 13002-1-AP

    IP result of anti-CUGBP1 (IP:13002-1-AP, 4ug; Detection:13002-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CUGBP1 Polyclonal antibody (13002-1-AP)

    IHC staining of human breast cancer using 13002-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13002-1-AP (CUGBP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CUGBP1 Polyclonal antibody (13002-1-AP)

    IHC staining of human skin cancer using 13002-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13002-1-AP (CUGBP1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CUGBP1 Polyclonal antibody (13002-1-AP)

    IHC staining of human breast cancer using 13002-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13002-1-AP (CUGBP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using CUGBP1 Polyclonal antibody (13002-1-AP)

    IHC staining of human testis using 13002-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13002-1-AP (CUGBP1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CUGBP1 Polyclonal antibody (13002-1-AP)

    IHC staining of human testis using 13002-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13002-1-AP (CUGBP1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CUGBP1 Polyclonal antibody (13002-1-AP)

    IHC staining of human skin cancer using 13002-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13002-1-AP (CUGBP1 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CUGBP1 Polyclonal antibody (13002-1-AP)

    IF Staining of HeLa using 13002-1-AP

    Immunofluorescent analysis of Hela cells, using CUGBP1 antibody 13002-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, NIH/3T3 cells, HT-1080 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human skin cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13002-1-AP targets CUGBP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUGBP1 fusion protein Ag3677
Full Name CUG triplet repeat, RNA binding protein 1
Calculated Molecular Weight 483 aa, 52 kDa
Observed Molecular Weight 51-55 kDa
GenBank Accession NumberBC031079
Gene Symbol CUGBP1
Gene ID (NCBI) 10658
RRIDAB_10642947
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CUG triplet repeat RNA binding protein 1 (CUGBP1) is a member of the CUGBP Elav-like family (CELF), which plays an important role in cardiac development and function (PMID: 17130239). CUGBP1 has also been shown to localize to cytoplasmic stress granules when cells are stressed.

Protocols

Product Specific Protocols
WB protocol for CUGBP1 antibody 13002-1-APDownload protocol
IHC protocol for CUGBP1 antibody 13002-1-APDownload protocol
IF protocol for CUGBP1 antibody 13002-1-APDownload protocol
IP protocol for CUGBP1 antibody 13002-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Hum Mol Genet

CGG repeats in RNA modulate expression of TDP-43 in mouse and fly models of fragile X tremor ataxia syndrome.

Authors - Jocelyn N Galloway
View Article
humanWB

Carcinogenesis

RNA-binding protein CUGBP1 controls the differential INSR splicing in molecular subtypes of breast cancer cells and affects cell aggressiveness.

Authors - Gena Huang
  • KD Validated
View Article
humanWB

Sci Rep

DLX6-AS1 activated by H3K4me1 enhanced secondary cisplatin resistance of lung squamous cell carcinoma through modulating miR-181a-5p/miR-382-5p/CELF1 axis.

Authors - Xu Zhao
  • KD Validated
View Article
human,mouseWB

Bioengineered

Guizhi Fuling pill attenuates liver fibrosis in vitro and in vivo via inhibiting TGF-β1/Smad2/3 and activating IFN-γ/Smad7 signaling pathways.

Authors - Zhongliang Liu
View Article