• Featured Product
  • KD/KO Validated

CXCR3B-specific Monoclonal antibody

CXCR3B-specific Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2D6

Cat no : 60065-1-Ig

Synonyms

CD183, CKR L2, CMKAR3, CXC R3, CXCR 3, CXCR3, CXCR3A, CXCR3B, G protein coupled receptor 9, GPR9, IP 10 receptor, IP10 R, Mig R, MigR



Tested Applications

Positive WB detected inHela cells, U-937 cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inU-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60065-1-Ig targets CXCR3B-specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name chemokine (C-X-C motif) receptor 3
Calculated molecular weight 46 kDa
Observed molecular weight 40 kDa+43 kDa
GenBank accession numberAF469635
Gene symbol CXCR3
Gene ID (NCBI) 2833
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. Binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as functional receptor for CXCL4 (PMID:12782716). This monoclonal antibody is specific to CXCR3B.

Protocols

Product Specific Protocols
WB protocol for CXCR3B-specific antibody 60065-1-IgDownload protocol
IHC protocol for CXCR3B-specific antibody 60065-1-IgDownload protocol
FC protocol for CXCR3B-specific antibody 60065-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Innate lymphocyte-induced CXCR3B-mediated melanocyte apoptosis is a potential initiator of T-cell autoreactivity in vitiligo.

Authors - Meri K Tulic
humanFC

Breast Cancer (Auckl)

The Chemokine Receptor CXCR3 Isoform B Drives Breast Cancer Stem Cells.

Authors - Namita Kundu
humanIF

Wound Repair Regen

Differential Regulation of Pericyte Function by the CXC Receptor 3.

Authors - Richard J Bodnar
humanWB

Hum Pathol

Overexpression of the chemokine receptor CXCR3 and its correlation with favorable prognosis in gastric cancer.

Authors - Min Hu
humanWB

Cytokine

CXCL11 promotes tumor progression by the biased use of the chemokine receptors CXCR3 and CXCR7.

Authors - Malte Puchert
human

Cell Signal

CXCL12-CXCL4 heterodimerization prevents CXCL12-driven breast cancer cell migration.

Authors - Khanh T P Nguyen