CXCR3B-specific Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CXCR3B-specific Monoclonal Antibody for WB, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B2D6

Cat no : 60065-1-PBS

Synonyms

CD183, CKR L2, CMKAR3, CXC R3, CXCR 3, CXCR3, CXCR3B, CXCR3B-specific, G protein coupled receptor 9, GPR9, IP 10 receptor, IP10 R, Mig R, MigR

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Validation Data Gallery

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  WB analysis of MOLT-4 using 60065-1-Ig (same clone as 60065-1-PBS)

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60065-1-Ig (same clone as 60065-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 60065-1-Ig (same clone as 60065-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 60065-1-Ig (same clone as 60065-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 60065-1-Ig (same clone as 60065-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60065-1-Ig (same clone as 60065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60065-1-Ig (same clone as 60065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60065-1-Ig (same clone as 60065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60065-1-Ig (same clone as 60065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

• 

  FC experiment of U-937 using 60065-1-Ig (same clone as 60065-1-PBS)

  1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human CXCR3B-specific (60065-1-Ig, Clone:1B2D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60065-1-PBS targets CXCR3B-specific in WB, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: chemokine (C-X-C motif) receptor 3
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: AF469635
• Gene Symbol: CXCR3
• Gene ID (NCBI): 2833
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. Binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as functional receptor for CXCL4 (PMID:12782716). This monoclonal antibody is specific to CXCR3B.