NOX2 Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 19013-1-AP

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Con: 20 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
mouse brain tissue, human plasma tissue, mouse heart tissue, Raji cells

Positive IHC detected in:
human skin tissue

Recommended dilution:
WB : 1:500-1:1000
IHC : 1:100-1:400

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
cytochrome b-245, beta polypeptide

Calculated molecular weight:
577aa,65 kDa

Observed molecular weight:
55 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
CYBB

Synonyms
CYBB, Cytochrome b 245 heavy chain, NOX2, p91 PHOX
Background

NOX2, also named as CYBB, CGD, 91-phox, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, cytochrome b-245 and beta polypeptide, is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. This full length protein has three glycosylation sites. CYBB is found in human cardiomyocytes as multiple bands:the signal between 55 and 65 kDa is probably the unglycosylated protein, because the predicted molecular weight of unglycosylated CYBB protein is approximately 58 kDa to 65 kDa in phagocytes and the bands around 80 kDa probably represent glycosylated CYBB, as has also been shown in human umbilical vein endothelial cells(PMID:12610097). In other reports, it also can be detected a band of 91 kDa(PMID:19965781). In our results, it reavels the bands of 55-65 kDa and 80 kDa besides a band of 140 kDa that we do not find the reason.


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