|Positive WB detected in||HeLa cells, HEK-293 cells, rat liver tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, NIH/3T3 cells, mouse kidney tissue, HepG2 cells, C2C12 cells|
|Positive IHC detected in||human colon cancer tissue, human breast cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10993-1-AP targets Cytochrome c in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||chicken, cow, hamsters, Hippospongia sp, human, mouse, rat, rice, sheep, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cytochrome c fusion protein Ag1455|
|Full Name||cytochrome c, somatic|
|Calculated molecular weight||12 kDa|
|Observed molecular weight||12-15 kDa|
|GenBank accession number||BC009578|
|Gene ID (NCBI)||54205|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Chi (Verified Customer) (09-26-2019)
The antibody works very well with good signal and low background.
Xiaoping (Verified Customer) (10-22-2018)
The signal is good and the size is between 10-15kDa.