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Cytochrome c Polyclonal antibody
Cytochrome c Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
223
Cat no : 10993-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, rat liver tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, NIH/3T3 cells, mouse kidney tissue, HepG2 cells, C2C12 cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 210 publications below |
| IHC | See 8 publications below |
| IF | See 6 publications below |
Product Information
10993-1-AP targets Cytochrome c in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | chicken, cow, hamsters, Hippospongia sp, human, mouse, rat, rice, sheep, swine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cytochrome c fusion protein Ag1455 |
| Full Name | cytochrome c, somatic |
| Calculated molecular weight | 12 kDa |
| Observed molecular weight | 12-15 kDa |
| GenBank accession number | BC009578 |
| Gene symbol | CYCS |
| Gene ID (NCBI) | 54205 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytochrome c antibody 10993-1-AP | Download protocol |
| IHC protocol for Cytochrome c antibody 10993-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Pharmacol Protective Effects of Costunolide Against D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice. | ||
Am J Chin Med Ferulic Acid Exerts Anti-apoptotic Effects against Ischemic Injury by Activating HSP70/Bcl-2- and HSP70/Autophagy-Mediated Signaling after Permanent Focal Cerebral Ischemia in Rats. | ||
Neural Regen Res Neuroprotective effects of rapamycin on spinal cord injury in rats by increasing autophagy and Akt signaling. | ||
J Biophotonics Photobiomodulation Preconditioning Prevents Cognitive Impairment in a Neonatal Rat Model of Hypoxia-ischemia. | ||
Stress Regulating glycolysis, the TLR4 signal pathway and expression of RBM3 in mouse liver in response to acute cold exposure. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Chi (Verified Customer) (09-26-2019) | The antibody works very well with good signal and low background.
 |
FH Xiaoping (Verified Customer) (10-22-2018) | The signal is good and the size is between 10-15kDa.
 |
