|Positive WB detected in||human brain tissue, L02 cells|
|Positive IP detected in||L02 cells|
|Positive IHC detected in||human hepatocirrhosis tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21555-1-AP is CYP26B1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CYP26B1 fusion protein Ag15368|
|Full Name||cytochrome P450, family 26, subfamily B, polypeptide 1|
|Calculated molecular weight||512 aa, 58 kDa|
|Observed molecular weight||58-60 kDa|
|GenBank accession number||BC069443|
|Gene ID (NCBI)||56603|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CYP26B1(Cytochrome P450 26B1) is involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation.It has 3 isoforms produced by alternative splicing.Cyp26b1 deficiency induces pronounced apoptosis in the developing limb and delayed chondrocyte maturation(PMID:15030763).Defects in CYP26B1 are the cause of radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA).
|Product Specific Protocols|
|WB protocol for CYP26B1 antibody 21555-1-AP||Download protocol|
|IHC protocol for CYP26B1 antibody 21555-1-AP||Download protocol|
|IF protocol for CYP26B1 antibody 21555-1-AP||Download protocol|
|IP protocol for CYP26B1 antibody 21555-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mol Reprod Dev
CYP26B1 declines postnatally in Sertoli cells independently of androgen action in the mouse testis.
J Alzheimers Dis
Decay in Retinoic Acid Signaling in Varied Models of Alzheimer's Disease and In-Vitro Test of Novel Retinoic Acid Receptor Ligands (RAR-Ms) to Regulate Protective Genes.
Control of retinoid levels by CYP26B1 is important for lymphatic vascular development in the mouse embryo.
De novo-synthesized retinoic acid in ovarian antral follicles enhances FSH-mediated ovarian follicular cell differentiation and female fertility.
Front Mol Neurosci
Transcriptomics of Environmental Enrichment Reveals a Role for Retinoic Acid Signaling in Addiction.
Increased Retinoic Acid Catabolism in Olfactory Sensory Neurons Activates Dormant Tissue-Specific Stem Cells and Accelerates Age-Related Metaplasia.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Daniel (Verified Customer) (02-06-2020)
The antibody stains a subset of proximal tubulues.