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  • KD/KO Validated

CYP26B1 Polyclonal antibody

CYP26B1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21555-1-AP

Synonyms

CYP26A2, CYP26B1, Cytochrome P450 26A2, Cytochrome P450 26B1, Cytochrome P450RAI 2, P450RAI 2, P450RAI2



Tested Applications

Positive WB detected inhuman brain tissue, L02 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21555-1-AP is CYP26B1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP26B1 fusion protein Ag15368
Full Name cytochrome P450, family 26, subfamily B, polypeptide 1
Calculated molecular weight 512 aa, 58 kDa
Observed molecular weight 58-60 kDa
GenBank accession numberBC069443
Gene symbol CYP26B1
Gene ID (NCBI) 56603
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP26B1(Cytochrome P450 26B1) is involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation.It has 3 isoforms produced by alternative splicing.Cyp26b1 deficiency induces pronounced apoptosis in the developing limb and delayed chondrocyte maturation(PMID:15030763).Defects in CYP26B1 are the cause of radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA).

Protocols

Product Specific Protocols
WB protocol for CYP26B1 antibody 21555-1-APDownload protocol
IHC protocol for CYP26B1 antibody 21555-1-APDownload protocol
IF protocol for CYP26B1 antibody 21555-1-APDownload protocol
IP protocol for CYP26B1 antibody 21555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Mol Reprod Dev

CYP26B1 declines postnatally in Sertoli cells independently of androgen action in the mouse testis.

Authors - Nadia Y Edelsztein
mouseWB

J Alzheimers Dis

Decay in Retinoic Acid Signaling in Varied Models of Alzheimer's Disease and In-Vitro Test of Novel Retinoic Acid Receptor Ligands (RAR-Ms) to Regulate Protective Genes.

Authors - Thabat Khatib
mouseIHC

Dev Biol

Control of retinoid levels by CYP26B1 is important for lymphatic vascular development in the mouse embryo.

Authors - Josephine Bowles
humanIF

Endocrinology

De novo-synthesized retinoic acid in ovarian antral follicles enhances FSH-mediated ovarian follicular cell differentiation and female fertility.

Authors - Tomoko Kawai

Front Mol Neurosci

Transcriptomics of Environmental Enrichment Reveals a Role for Retinoic Acid Signaling in Addiction.

Authors - Yafang Zhang
mouseIF

J Neurosci

Increased Retinoic Acid Catabolism in Olfactory Sensory Neurons Activates Dormant Tissue-Specific Stem Cells and Accelerates Age-Related Metaplasia.

Authors - Sofia Håglin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Daniel (Verified Customer) (02-06-2020)

The antibody stains a subset of proximal tubulues.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: mouse kidney
CYP26B1 Antibody Immunofluorescence, validation (1:200 dilution) in mouse kidney (Cat no:21555-1-AP)