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DCLRE1B Polyclonal antibody

DCLRE1B Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 13203-1-AP

Synonyms

5 exonuclease Apollo, APOLLO, DCLRE1B, hSNM1B, SNM1 homolog B, SNM1B, SNMIB

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using DCLRE1B Polyclonal antibody (13203-1-AP)

    WB analysis of Jurkat using 13203-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13203-1-AP (DCLRE1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using DCLRE1B Polyclonal antibody (13203-1-AP)

    WB analysis of HepG2 using 13203-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13203-1-AP (DCLRE1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DCLRE1B Polyclonal antibody (13203-1-AP)

    IF Staining of HepG2 using 13203-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13203-1-AP (DCLRE1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13203-1-AP targets DCLRE1B in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCLRE1B fusion protein Ag3945
Full Name DNA cross-link repair 1B (PSO2 homolog, S. cerevisiae)
Calculated Molecular Weight 532 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC029687
Gene Symbol DCLRE1B
Gene ID (NCBI) 64858
RRIDAB_2292695
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interstrand cross-links (ICLs) is an extremely cytotoxic form of DNA damage that covalently tethers the two strands of the double helix, which inhibits fundamental nuclear functions, including transcription and DNA replication [PMID:21701511]. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links [PMID:10848582]. Furthermore, DCLRE1B was identified as a TRF2-interacting protein and, has a role in telomere maintenance in association with the Shelterin complex, preventing non-homologous end-joining-mediated chromosome fusions protecting leading strand telomeres [PMID:20619712].

Protocols

Product Specific Protocols
WB protocol for DCLRE1B antibody 13203-1-APDownload protocol
IF protocol for DCLRE1B antibody 13203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Struct Mol Biol

Fanconi anemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mouse development

Authors - Nazareno Bona
View Article