DCP1A Polyclonal antibody

DCP1A Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22373-1-AP

Synonyms

DCP1A, HSA275986, mRNA decapping enzyme 1A, Nbla00360, SMAD4IP1, SMIF, Transcription factor SMIF



Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, mouse lung tissue, Jurkat cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22373-1-AP is DCP1A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCP1A fusion protein Ag18027
Full Name DCP1 decapping enzyme homolog A (S. cerevisiae)
Calculated molecular weight 582 aa, 63 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC007439
Gene symbol DCP1A
Gene ID (NCBI) 55802
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.

Protocols

Product Specific Protocols
WB protocol for DCP1A antibody 22373-1-APDownload protocol
IHC protocol for DCP1A antibody 22373-1-APDownload protocol
IP protocol for DCP1A antibody 22373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols