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DDX3 Polyclonal antibody

Name: DDX3 Polyclonal antibody
Brand: Proteintech
SKU: 11115-1-AP
Rating: 5.00 (1 reviews)

DDX3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 11115-1-AP

Synonyms

DBX, DDX14, DDX3, DDX3X, DEAD box, X isoform, Helicase like protein 2, HLP2

Validation Data Gallery

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AllWBIPIHCIFFC

WB analysis using 11115-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 11115-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11115-1-AP
WB result of DDX3 antibody (11115-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HepG2 cells.
WB analysis of HeLa using 11115-1-AP
WB result of DDX3 antibody (11115-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HeLa cells.
WB analysis using 11115-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11115-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 11115-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 11115-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11115-1-AP
IP result of anti-DDX3 (IP:11115-1-AP, 4ug; Detection:11115-1-AP 1:300) with HeLa cells lysate 2480ug.
IHC staining of human malignant melanoma using 11115-1-AP
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 11115-1-AP (DDX3 antibody) at dilution of 1:200 (under 40x lens)..
IHC staining of human malignant melanoma using 11115-1-AP
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 11115-1-AP (DDX3 antibody) at dilution of 1:200 (under 10x lens)..
IHC staining of human cervical cancer using 11115-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11115-1-AP (DDX3 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HepG2 using 11115-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDX3 antibody (11115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 11115-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX3 antibody (11115-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 11115-1-AP
Immunofluorescent analysis of HepG2 cells using 11115-1-AP (DDX3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
FC experiment of HeLa using 11115-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DDX3 (11115-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 11115-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, NIH/3T3 cells, HepG2 cells, mouse liver tissue, HT-1080 cells, mouse brain tissue, PC-12 cells, K-562 cells, C6 cells, rat brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human malignant melanoma tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells
Positive FC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:200-1:800
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 22 publications below
IHC	See 4 publications below
IF	See 7 publications below
CoIP	See 4 publications below
RIP	See 2 publications below

Product Information

11115-1-AP targets DDX3 in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DDX3 fusion protein Ag1614
Full Name	DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
Calculated Molecular Weight	73 kDa
Observed Molecular Weight	73 kDa
GenBank Accession Number	BC011819
Gene Symbol	DDX3X
Gene ID (NCBI)	1654
RRID	AB_10896499
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX3X, also named as DBX, DDX3 and HLP2, belongs to the DEAD box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73 kDa is the target band. 60-65 kDa is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.

Protocols

Product Specific Protocols
WB protocol for DDX3 antibody 11115-1-AP	Download protocol
IHC protocol for DDX3 antibody 11115-1-AP	Download protocol
IF protocol for DDX3 antibody 11115-1-AP	Download protocol
IP protocol for DDX3 antibody 11115-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	IF	Neuron Pathogenic DDX3X Mutations Impair RNA Metabolism and Neurogenesis during Fetal Cortical Development. Authors - Ashley L Lennox View Article
human	WB	Neuron CRISPR-Cas9 Screens Identify the RNA Helicase DDX3X as a Repressor of C9ORF72 (GGGGCC)n Repeat-Associated Non-AUG Translation. Authors - Weiwei Cheng KD Validated View Article
human	WB	Pharmacol Res HPV E7-drived ALKBH5 promotes cervical cancer progression by modulating m6A modification of PAK5 Authors - Fu-Chun Huo KD Validated View Article
mouse	WB,IF,CoIP	Ecotoxicol Environ Saf Aluminum exposure induces central nervous system impairment via activating NLRP3-medicated pyroptosis pathway Authors - Wudi Hao View Article
	WB,IF	J Virol DDX3X Is Hijacked by Snakehead Vesiculovirus Phosphoprotein To Facilitate Virus Replication via Stabilization of the Phosphoprotein Authors - Congran Bei View Article
human	WB,IF	Oncotarget (DEAD)-box RNA helicase 3 modulates NF-κB signal pathway by controlling the phosphorylation of PP2A-C subunit. Authors - Xin Wang KD Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Shannon (Verified Customer) (07-19-2022)

Worked well, also binds to different isoforms/protein dimers

Applications: Western Blot, Immunoprecipitation
Primary Antibody Dilution: 1:2000
Cell Tissue Type: Jurkat T-Cells

DDX3 Antibody Western Blot,Immunoprecipitation validation (1:2000 dilution) in Jurkat T-Cells (Cat no:11115-1-AP)

DDX3 Polyclonal antibody

DDX3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 11115-1-AP

WB analysis using 11115-1-AP

WB analysis of HepG2 using 11115-1-AP

WB analysis of HeLa using 11115-1-AP

WB analysis using 11115-1-AP

WB analysis of mouse liver using 11115-1-AP

WB analysis of NIH/3T3 using 11115-1-AP

WB analysis of HT-1080 using 11115-1-AP

IP experiment of HeLa using 11115-1-AP

IHC staining of human malignant melanoma using 11115-1-AP

IHC staining of human malignant melanoma using 11115-1-AP

IHC staining of human cervical cancer using 11115-1-AP

IF Staining of HepG2 using 11115-1-AP

IF Staining of HepG2 using 11115-1-AP

IF Staining of HepG2 using 11115-1-AP

FC experiment of HeLa using 11115-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Pathogenic DDX3X Mutations Impair RNA Metabolism and Neurogenesis during Fetal Cortical Development.

CRISPR-Cas9 Screens Identify the RNA Helicase DDX3X as a Repressor of C9ORF72 (GGGGCC)n Repeat-Associated Non-AUG Translation.

HPV E7-drived ALKBH5 promotes cervical cancer progression by modulating m6A modification of PAK5

Aluminum exposure induces central nervous system impairment via activating NLRP3-medicated pyroptosis pathway

DDX3X Is Hijacked by Snakehead Vesiculovirus Phosphoprotein To Facilitate Virus Replication via Stabilization of the Phosphoprotein

(DEAD)-box RNA helicase 3 modulates NF-κB signal pathway by controlling the phosphorylation of PP2A-C subunit.

Reviews