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DEPDC7 Polyclonal antibody

DEPDC7 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 26517-1-AP

Synonyms

DEP domain containing 7, DEPDC7, Protein TR2/D15, TR2

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using DEPDC7 Polyclonal antibody (26517-1-AP)

    WB analysis of HepG2 using 26517-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26517-1-AP (DEPDC7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DEPDC7 Polyclonal antibody (26517-1-AP)

    WB analysis of HeLa using 26517-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26517-1-AP (DEPDC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DEPDC7 Polyclonal antibody (26517-1-AP)

    IF Staining of HepG2 using 26517-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 26517-1-AP (DEPDC7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26517-1-AP is DEPDC7 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DEPDC7 fusion protein Ag24094
Full Name DEP domain containing 7
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC030970
Gene Symbol DEPDC7
Gene ID (NCBI) 91614
RRIDAB_2880539
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEPDC7 (DEP domain-containing protein 7), also known as protein TR2/D15, is a 511 amino acid protein belonging to the DEPDC7 family. DEPDC7 contains one DEP domain and is highly expressed in liver, with lower levels in kidney. DEPDC7 exists as two isoforms produced by alternative splicing events. The gene encoding DEPDC7 maps to human chromosome 11p13 and mouse chromosome 2 E2. With approximately 135 million base pairs and 1,400 genes, chromosome 11 makes up around 4% of human genomic DNA and is considered a gene and disease association dense chromosome. DEPDC7 involves the regulation of small GTPase mediated signal transduction.

Protocols

Product Specific Protocols
WB protocol for DEPDC7 antibody 26517-1-APDownload protocol
IF protocol for DEPDC7 antibody 26517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols