- Featured Product
- KD/KO Validated
DGCR8 C-terminal Polyclonal antibody
DGCR8 C-terminal Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, RIP, IP, IHC, IF, FC, ChIP,ELISA
Conjugate
Unconjugated
Cat no : 10996-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, A431 cells, Jurkat cells, HeLa cells, mouse testis tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | SH-SY5Y cells |
| Positive FC detected in | HeLa cells, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 11 publications below |
| WB | See 60 publications below |
| IHC | See 5 publications below |
| IF | See 6 publications below |
| IP | See 2 publications below |
| ChIP | See 1 publications below |
| RIP | See 1 publications below |
Product Information
10996-1-AP targets DGCR8 C-terminal in WB, RIP, IP, IHC, IF, FC, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DGCR8 C-terminal fusion protein Ag1429 |
| Full Name | DiGeorge syndrome critical region gene 8 |
| Calculated molecular weight | 773 aa, 86 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | BC009323 |
| Gene symbol | DGCR8 |
| Gene ID (NCBI) | 54487 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa (PMID: 18469815).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DGCR8 C-terminal antibody 10996-1-AP | Download protocol |
| IHC protocol for DGCR8 C-terminal antibody 10996-1-AP | Download protocol |
| IF protocol for DGCR8 C-terminal antibody 10996-1-AP | Download protocol |
| IP protocol for DGCR8 C-terminal antibody 10996-1-AP | Download protocol |
| FC protocol for DGCR8 C-terminal antibody 10996-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol Microprocessor mediates transcriptional termination of long noncoding RNA transcripts hosting microRNAs.
| ||
Int J Gen Med Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I. | ||
Cell Stem Cell Principles of signaling pathway modulation for enhancing human naive pluripotency induction. | ||
Bone Deficiency of DGCR8 increases bone formation through downregulation of miR-22 expression.
| ||
