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KD/KO Validated

DGCR8 C-terminal Polyclonal antibody

Name: DGCR8 C-terminal Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 10996-1-AP

DGCR8 C-terminal Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, FC, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10996-1-AP

Synonyms

C22orf12, DGCR8, DGCRK6, Gy1

Validation Data Gallery

WB analysis of Jurkat cells using 10996-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 cells using 10996-1-AP
IP Result of anti-DGCR8 C-terminal (IP:10996-1-AP, 3ug; Detection:10996-1-AP 1:800) with HEK-293 cells lysate 2700ug.
IHC staining of human breast cancer tissue using 10996-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human breast cancer tissue using 10996-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:100 (under 40x lens).
IF staining of SH-SY5Y cells using 10996-1-AP
Immunofluorescent analysis of SH-SY5Y cells, using DGCR8 antibody 10996-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
FC experiment of HeLa cells using 10996-1-AP
1X10^6 HeLa cells were stained with 0.2ug DGCR8 C-terminal antibody (10996-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Cat No. 10996-1-AP

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Tested Applications

Positive WB detected in	Jurkat cells, A431 cells, HEK-293 cells, HeLa cells, mouse testis tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human breast cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	SH-SY5Y cells
Positive FC detected in	HeLa cells, SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)	IHC : 1:50-1:200
Immunofluorescence (IF)	IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 10 publications below
WB	See 52 publications below
IHC	See 5 publications below
IF	See 4 publications below
IP	See 2 publications below
ChIP	See 1 publications below
RIP	See 1 publications below

Product Information

10996-1-AP targets DGCR8 C-terminal in WB, RIP, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DGCR8 C-terminal fusion protein Ag1429
Full Name	DiGeorge syndrome critical region gene 8
Calculated molecular weight	773 aa, 86 kDa
Observed molecular weight	120 kDa
GenBank accession number	BC009323
Gene symbol	DGCR8
Gene ID (NCBI)	54487
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa (PMID: 18469815).

Protocols

Product Specific Protocols
WB protocol for DGCR8 C-terminal antibody 10996-1-AP	Download protocol
IHC protocol for DGCR8 C-terminal antibody 10996-1-AP	Download protocol
IF protocol for DGCR8 C-terminal antibody 10996-1-AP	Download protocol
IP protocol for DGCR8 C-terminal antibody 10996-1-AP	Download protocol
FC protocol for DGCR8 C-terminal antibody 10996-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse		BMC Biol Biological and RNA regulatory function of MOV10 in mammalian germ cells. Authors - Kaiqiang Fu View Article
human	WB	Nat Struct Mol Biol Microprocessor mediates transcriptional termination of long noncoding RNA transcripts hosting microRNAs. Authors - Ashish Dhir KD Validated View Article
human	WB,IHC	Int J Gen Med Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I. Authors - Emad Babakhanzadeh View Article
mouse	WB,IHC,IF	Bone Deficiency of DGCR8 increases bone formation through downregulation of miR-22 expression. Authors - Young-Jin Choi KD Validated View Article
human	WB	Methods Mol Biol High-Throughput Characterization of Primary microRNA Transcripts. Authors - Tsung-Cheng Chang View Article
human	WB	Genes Cancer Impaired MicroRNA Processing Facilitates Breast Cancer Cell Invasion by Upregulating Urokinase-Type Plasminogen Activator Expression. Authors - Noh Hyangsoon H View Article