DNASE1L3 Polyclonal antibody

DNASE1L3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11330-1-AP

Synonyms

Deoxyribonuclease gamma, deoxyribonuclease I like 3, DHP2, DNAS1L3, DNase gamma, DNase I homolog protein DHP2, DNase I like 3, DNASE1L3, Liver and spleen DNase, LS DNase, LSD



Tested Applications

Positive WB detected inHEK-293 cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:600
Sample-dependent, check data in validation data gallery

Product Information

11330-1-AP targets DNASE1L3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNASE1L3 fusion protein Ag1873
Full Name deoxyribonuclease I-like 3
Calculated molecular weight 305 aa, 36 kDa
Observed molecular weight 33-38 kDa
GenBank accession numberBC015831
Gene symbol DNASE1L3
Gene ID (NCBI) 1776
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Deoxyribonuclease 1-like 3 (DNASE1L3, DNase gamma, DNase Y, LS-DNase) is member of a DNASE1 protein family that is identified by similar biochemical properties such as Ca2+/Mg2+ dependency and an optimal pH of about 7.0 as well as by a high similarity in their nucleic acid and amino acid sequences [PMID:15796714]. It is an endonuclease and cleaves double-stranded DNA (and single-stranded DNA) producing 3’-OH/5’-P ends, which is Ca2+/Mg2+-dependent and inhibited by Zn2+, but not by monomeric actin [PMID:9665719]. In addition, it has been suggested to be involved in apoptotic DNA fragmentation of thymocytes neuronal PC12 cells, C2C12 myoblasts and of cell lines expressing the recombinant nuclease [PMID: 7957253, 9307016]

Protocols

Product Specific Protocols
WB protocol for DNASE1L3 antibody 11330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols