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DUSP22 Polyclonal antibody

DUSP22 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 16514-1-AP

Synonyms

DUSP22, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using DUSP22 Polyclonal antibody (16514-1-AP)

    WB analysis of mouse heart using 16514-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16514-1-AP (DUSP22 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using DUSP22 Polyclonal antibody (16514-1-AP)

    WB analysis of mouse kidney using 16514-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16514-1-AP (DUSP22 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DUSP22 Polyclonal antibody (16514-1-AP)

    IHC staining of human lung cancer using 16514-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 16514-1-AP (DUSP22 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DUSP22 Polyclonal antibody (16514-1-AP)

    IHC staining of human lung cancer using 16514-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 16514-1-AP (DUSP22 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse heart tissue, mouse kidney tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:600
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16514-1-AP targets DUSP22 in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DUSP22 fusion protein Ag9734
Full Name dual specificity phosphatase 22
Calculated Molecular Weight 184 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC022847
Gene Symbol DUSP22
Gene ID (NCBI) 56940
RRIDAB_2095013
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DUSP22(Dual specificity protein phosphatase 22) is also named as JSP1, LMWDSP2, MKPX. It has been demonstrated to belong to a new subgroup of small DSPs anchored at the membranes by an N-terminal myristic acid moiety and DUSP22 play a regulatory role in the JNK or p38 MAPK pathways(PMID:17384676). It has 2 isoforms produced by alternative splicing with the molecular weight of 21 kDa and 23 kDa.

Protocols

Product Specific Protocols
WB protocol for DUSP22 antibody 16514-1-APDownload protocol
IHC protocol for DUSP22 antibody 16514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

FASEB J

DUSP22 suppresses prostate cancer proliferation by targeting the EGFR-AR axis.

Authors - Hsiu-Ping Lin
View Article
humanWB

Oncotarget

Molecular alterations and tumor suppressive function of the DUSP22 (Dual Specificity Phosphatase 22) gene in peripheral T-cell lymphoma subtypes.

Authors - Pierre Mélard
View Article