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  • Featured Product
  • KD/KO Validated

DVL2 Polyclonal antibody

DVL2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12037-1-AP

Synonyms

Dishevelled 2, DSH homolog 2, DVL2

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using DVL2 Polyclonal antibody (12037-1-AP)

    WB analysis of HepG2 cells using 12037-1-AP

    WB result of DVL2 antibody (12037-1-AP, 1:1000) with siDVL2 and si-control transfected cells.

  • Western Blot (WB) analysis of various lysates using DVL2 Polyclonal antibody (12037-1-AP)

    WB analysis using 12037-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using DVL2 Polyclonal antibody (12037-1-AP)

    WB analysis of MDA-MB-453s using 12037-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using DVL2 Polyclonal antibody (12037-1-AP)

    WB analysis of MCF-7 using 12037-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse thymus tissue using DVL2 Polyclonal antibody (12037-1-AP)

    WB analysis of mouse thymus using 12037-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using DVL2 Polyclonal antibody (12037-1-AP)

    IP experiment of MCF-7 using 12037-1-AP

    IP result of anti-DVL2 (IP:12037-1-AP, 4ug; Detection:12037-1-AP 1:4000) with MCF-7 cells lysate 640 ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DVL2 Polyclonal antibody (12037-1-AP)

    IHC staining of human lung cancer using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DVL2 Polyclonal antibody (12037-1-AP)

    IHC staining of human lung cancer using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using DVL2 Polyclonal antibody (12037-1-AP)

    IHC staining of human gliomas using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using DVL2 Polyclonal antibody (12037-1-AP)

    IHC staining of human gliomas using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using DVL2 Polyclonal antibody (12037-1-AP)

    IHC staining of human gliomas using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using DVL2 Polyclonal antibody (12037-1-AP)

    IHC staining of human gliomas using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DVL2 Polyclonal antibody (12037-1-AP)

    IHC staining of human prostate cancer using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DVL2 Polyclonal antibody (12037-1-AP)

    IHC staining of human prostate cancer using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using DVL2 Polyclonal antibody (12037-1-AP)

    IHC staining of mouse colon using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using DVL2 Polyclonal antibody (12037-1-AP)

    IHC staining of mouse colon using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using DVL2 Polyclonal antibody (12037-1-AP)

    IHC staining of rat colon using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using DVL2 Polyclonal antibody (12037-1-AP)

    IHC staining of rat colon using 12037-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DVL2 Polyclonal antibody (12037-1-AP)

    IF Staining of HepG2 using 12037-1-AP

    Immunofluorescent analysis of HepG2 cells using 12037-1-AP (DVL2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using DVL2 Polyclonal antibody (12037-1-AP)

    IF Staining of HCT 116 using 12037-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using DVL2 antibody (12037-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, mouse thymus tissue, MCF-7 cells, MDA-MB-453s cells, HepG2 cells, MDA-MB-231 cells, NIH/3T3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human gliomas tissue, human prostate cancer tissue, mouse colon tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHCT 116 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12037-1-AP targets DVL2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, Xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DVL2 fusion protein Ag2666
Full Name dishevelled, dsh homolog 2 (Drosophila)
Calculated Molecular Weight 736 aa, 79 kDa
Observed Molecular Weight 90-95 kDa
GenBank Accession NumberBC014844
Gene Symbol DVL2
Gene ID (NCBI) 1856
RRIDAB_2093330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DVL2 is member of the dishevelled (dsh) protein family. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein in a variety of cell lines and species. DVL2 participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. It promotes internalization and degradation of frizzled proteins upon Wnt signaling. It plays a role both in canonical and non-canonical Wnt signaling. It also Plays a role in the signal transduction pathways mediated by multiple Wnt genes.

Protocols

Product Specific Protocols
WB protocol for DVL2 antibody 12037-1-APDownload protocol
IHC protocol for DVL2 antibody 12037-1-APDownload protocol
IF protocol for DVL2 antibody 12037-1-APDownload protocol
IP protocol for DVL2 antibody 12037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Biol

Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling.

Authors - Andre Landin Malt
View Article
humanWB

Cell Death Dis

YAP triggers the Wnt/β-catenin signalling pathway and promotes enterocyte self-renewal, regeneration and tumorigenesis after DSS-induced injury.

Authors - Feihong Deng
View Article
humanWB

Development

The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning.

Authors - Verena Rauschenberger
View Article
mouseIHC

Front Cell Dev Biol

Non-Canonical Wnt Signaling Regulates Cochlear Outgrowth and Planar Cell Polarity via Gsk3β Inhibition.

Authors - Andre Landin Malt
View Article
humanWB,IF

FASEB J

Cyclin G2 upregulation impairs migration, invasion, and network formation through RNF123/Dvl2/JNK signaling in the trophoblast cell line HTR8/SVneo, a possible role in preeclampsia.

Authors - Manni Sun
View Article
humanWB, IP

Mol Biol Cell

Distinct functionality of Dishevelled isoforms on Ca2+/Calmodulin dependent protein kinase 2 (CamKII) in Xenopus gastrulation.

Authors - Marc Gentzel
View Article