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  • Featured Product
  • KD/KO Validated

EDIL3 Polyclonal antibody

EDIL3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12580-1-AP

Synonyms

DEL1, EDIL3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using EDIL3 Polyclonal antibody (12580-1-AP)

    WB analysis using 12580-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using EDIL3 Polyclonal antibody (12580-1-AP)

    WB analysis of HEK-293 using 12580-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using EDIL3 Polyclonal antibody (12580-1-AP)

    WB analysis of COLO 320 using 12580-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using EDIL3 Polyclonal antibody (12580-1-AP)

    WB analysis of mouse brain using 12580-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using EDIL3 Polyclonal antibody (12580-1-AP)

    WB analysis of HepG2 using 12580-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using EDIL3 Polyclonal antibody (12580-1-AP)

    WB analysis of mouse lung using 12580-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lung tissue using EDIL3 Polyclonal antibody (12580-1-AP)

    WB analysis of human lung using 12580-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EDIL3 Polyclonal antibody (12580-1-AP)

    WB analysis of HeLa using 12580-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using EDIL3 Polyclonal antibody (12580-1-AP)

    WB analysis of K-562 using 12580-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using EDIL3 Polyclonal antibody (12580-1-AP)

    IHC staining of human pancreas cancer using 12580-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12580-1-AP (EDIL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using EDIL3 Polyclonal antibody (12580-1-AP)

    IHC staining of human pancreas cancer using 12580-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12580-1-AP (EDIL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EDIL3 Polyclonal antibody (12580-1-AP)

    IHC staining of human lung cancer using 12580-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12580-1-AP (EDIL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EDIL3 Polyclonal antibody (12580-1-AP)

    IHC staining of human lung cancer using 12580-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12580-1-AP (EDIL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using EDIL3 Polyclonal antibody (12580-1-AP)

    IHC staining of human liver cancer using 12580-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 12580-1-AP (EDIL3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using EDIL3 Polyclonal antibody (12580-1-AP)

    IHC staining of human liver cancer using 12580-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 12580-1-AP (EDIL3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EDIL3 Polyclonal antibody (12580-1-AP)

    IF Staining of HeLa using 12580-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12580-1-AP (EDIL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, COLO 320 cells, mouse brain tissue, HepG2 cells, mouse lung tissue, human lung tissue, HeLa cells, K-562 cells
Positive IHC detected inhuman pancreas cancer tissue, human lung cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12580-1-AP targets EDIL3 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EDIL3 fusion protein Ag3274
Full Name EGF-like repeats and discoidin I-like domains 3
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52-55 kDa
GenBank Accession NumberBC030828
Gene Symbol EDIL3
Gene ID (NCBI) 10085
RRIDAB_2096219
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGF-like repeats and discoidin I-like domain-containing protein 3 (EDIL3), also named as DEL1 or integrin-biding protein DEL1, is a 52-kDa extracellular matrix protein produced by endothelial cells in embryos (PMID: 9420328). It is composed of three EGF repeats and two discoidin I-like domains. The second EGF repeat contains an RGD motif. EDIL3 promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. It may be involved in vascular remodeling during angiogenesis (PMID: 12840057; 14981004). EDIL3 has also been reported to be an endogenous inhibitor of inflammatory cell recruitment by interfering with the integrin LFA-1-dependent leukocyte-endothelial adhesion (PMID: 19008446).

Protocols

Product Specific Protocols
WB protocol for EDIL3 antibody 12580-1-APDownload protocol
IHC protocol for EDIL3 antibody 12580-1-APDownload protocol
IF protocol for EDIL3 antibody 12580-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Clin Invest

Secreted protein Del-1 regulates myelopoiesis in the hematopoietic stem cell niche.

Authors - Ioannis Mitroulis
  • KO Validated
View Article
humanELISA

Nat Commun

Antagonistic effects of IL-17 and D-resolvins on endothelial Del-1 expression through a GSK-3β-C/EBPβ pathway.

Authors - Tomoki Maekawa
View Article
humanWB,IHC

Oncotarget

Overexpressed EDIL3 predicts poor prognosis and promotes anchorage-independent tumor growth in human pancreatic cancer.

Authors - Shu-Heng Jiang
  • KD Validated
View Article

Thromb Haemost

Developmental endothelial locus-1 attenuates complement-dependent phagocytosis through inhibition of Mac-1-integrin.

Authors - Ioannis Mitroulis
View Article

Sci Rep

Inhibition of CDK9 as a therapeutic strategy for inflammatory arthritis.

Authors - Annelie Hellvard
View Article
humanWB, IHC

Mol Cancer

Elevated autocrine EDIL3 protects hepatocellular carcinoma from anoikis through RGD-mediated integrin activation.

Authors - Ming-Xuan Feng
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Guo-rong (Verified Customer) (08-30-2022)

It gives a clear and specific band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: ARPE-19
EDIL3 Antibody Western Blot validation (1:500 dilution) in ARPE-19 (Cat no:12580-1-AP)