EIF3J Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 10439-1-AP

Featured Product KD/KO validated

  • Print page
  • Download PDF

Be the first to review this product


-_-

Freight/Packing

Con: 33 μg/150 μl

Choose size:

Please visit your regions distributor:


Species specificity:
human, mouse, rat

Positive WB detected in:
HeLa cells, A549 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells, SKOV-3 cells

Positive IP detected in:
HeLa cells

Positive IHC detected in:
human breast cancer tissue

Positive IF detected in:
Hela cells

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
eukaryotic translation initiation factor 3, subunit J

Calculated molecular weight:
28 kDa

Observed molecular weight:
35 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
EIF3J

Synonyms
eIF 3 alpha, eIF3 alpha, eIF3 p35, EIF3J, EIF3S1
Background

EIF3 has a key role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex(PMID:17588516). The eIF3 complex stimulates several steps in the translation initiation pathway, including dissociation of 80S ribosomes into 40S and 60S subunits, binding of a ternary complex (TC) consisting of Met-tRNA , eIF2, and GTP to the small subunit (forming the 43S preinitiation complex) and recruitment of mRNA to the 43 S complex to produce the 48S complex (PMID:11560931) EIF3J was identified as a high copy suppressor of the temperature-sensitive (Ts-) phenotype of the rpg1-1 allele of TIF32/RPG1, encoding the largest subunit of yeast eIF3 (eIF3a) (PMID:10488093).


Back
to top