Tested Applications
| Positive WB detected in | PC-12 cells, C6 cells, fetal human brain tissue, HEK-293 cells, Neuro-2a cells, pig brain tissue, rat brain tissue, SH-SY5Y cells, U-251 cells, pig cerebellum tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue |
| Positive IHC detected in | mouse brain tissue, human brain tissue, rat brain tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66150-1-Ig targets NSE in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19106 Product name: Recombinant human ENO2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 2-300 aa of BC002745 Sequence: SIEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERELPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELVKEAIDKAGYTEKIVIGMDVAASEFYRDGKYDLDFKSPTDPSRYITGDQLGALYQDFVRDYPVVSIEDPFDQDD Predict reactive species |
| Full Name | enolase 2 (gamma, neuronal) |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC002745 |
| Gene Symbol | NSE |
| Gene ID (NCBI) | 2026 |
| RRID | AB_2881546 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P09104-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for NSE antibody 66150-1-Ig | Download protocol |
| IF protocol for NSE antibody 66150-1-Ig | Download protocol |
| IHC protocol for NSE antibody 66150-1-Ig | Download protocol |
| WB protocol for NSE antibody 66150-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NSE Monoclonal antibody (6F8G3), catalog number 66150-1-Ig, has 38 citations across journals including Nature Cell Biology, Nature Communications, Redox Biology, Cell Death & Disease, Advanced Science, Cell Reports, Cancer Letters, Oncogenesis, Journal of Pathology, Scientific Reports, and others. Research fields span prostate cancer, pancreatic cancer, lung cancer, renal cell carcinoma, colorectal cancer, endometrial cancer, neuroinflammation, cerebral ischemia/reperfusion, traumatic brain injury, epilepsy, spinal cord injury, glycolytic metabolism, and enolase biology.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Prostate lineage-specific metabolism governs luminal differentiation and response to antiandrogen treatment | ||
Nat Commun Circulating extracellular vesicle microRNAs mediate immune modulation of social behavior in male mice. | ||
J Exp Clin Cancer Res PAX6 promotes neuroendocrine phenotypes of prostate cancer via enhancing MET/STAT5A-mediated chromatin accessibility | ||
Adv Sci (Weinh) Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer | ||
Cell Death Dis Lactate transmission from hypoxic tumor cells promotes macrophage senescence and M2 polarization via the DNMT1-NHE7 axis to accelerate endometrial cancer progression. |
Reviews
What customers say
One reviewer (Morgan W.) used this antibody at a 1:500 dilution for Western Blot on OV90 Ovarian Cancer Cells and rated it 4 out of 5 stars. They reported that overall it worked well and the trend was clear, though they noted some extra bands that made the result look a little dirty. The reviewer was not certain about the cause of the additional bands but found the antibody functional for their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Morgan (Verified Customer) (10-26-2021) | Overall it worked well. It looked a little dirty with some "extra" bands, but I'm not exactly sure why. The trend is still clear though.
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