NSE Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

NSE Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6F8G3

Cat no : 66150-1-PBS

Synonyms

ENO2, Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE

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Validation Data Gallery

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  WB analysis using 66150-1-Ig (same clone as 66150-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 66150-1-Ig (same clone as 66150-1-PBS)

  WB result of NSE antibody (66150-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSE transfected U-251 cells. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 66150-1-Ig (same clone as 66150-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66150-1-Ig (same clone as 66150-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 66150-1-Ig (same clone as 66150-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 66150-1-Ig (same clone as 66150-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 66150-1-Ig (same clone as 66150-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66150-1-Ig (same clone as 66150-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66150-1-Ig (same clone as 66150-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66150-1-Ig (same clone as 66150-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66150-1-Ig (same clone as 66150-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66150-1-Ig (NSE antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66150-1-Ig (NSE antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSE antibody (66150-1-Ig, Clone: 6F8G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunofluorescent analysis of SH-SY5Y cells using 66150-1-Ig (NSE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSE antibody (66150-1-Ig, Clone: 6F8G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 66150-1-Ig (same clone as 66150-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NSE (66150-1-Ig, Clone:6F8G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66150-1-PBS targets NSE in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NSE fusion protein Ag19106
• Full Name: enolase 2 (gamma, neuronal)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC002745
• Gene Symbol: ENO2
• Gene ID (NCBI): 2026
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.