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ENO3 Polyclonal antibody

ENO3 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16421-1-AP

Synonyms

Beta enolase, ENO3, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using ENO3 Polyclonal antibody (16421-1-AP)

    WB analysis of human skeletal muscle using 16421-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16421-1-AP (ENO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ENO3 Polyclonal antibody (16421-1-AP)

    WB analysis of human brain using 16421-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16421-1-AP (ENO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ENO3 Polyclonal antibody (16421-1-AP)

    IF Staining of HeLa using 16421-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 16421-1-AP (ENO3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman skeletal muscle tissue, human brain tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

16421-1-AP targets ENO3 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENO3 fusion protein Ag9613
Full Name enolase 3 (beta, muscle)
Calculated Molecular Weight 434 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC017249
Gene Symbol ENO3
Gene ID (NCBI) 2027
RRIDAB_2099464
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13).

Protocols

Product Specific Protocols
WB protocol for ENO3 antibody 16421-1-APDownload protocol
IF protocol for ENO3 antibody 16421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta Mol Cell Res

Phospholipase A2-activating protein induces mitophagy trough anti-apoptotic MCL1-mediated NLRX1 oligomerization

Authors - Zhilong Zheng
View Article
mouseWB

Mol Cell Proteomics

Extended Multiplexing of Tandem Mass Tags (TMT) Labeling Reveals Age and High Fat Diet Specific Proteome Changes in Mouse Epididymal Adipose Tissue.

Authors - Deanna L Plubell
View Article
ratWB

Cell Physiol Biochem

Gender-dimorphic regulation of skeletal muscle proteins in streptozotocin-induced diabetic rats.

Authors - Choi Minji M
View Article