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CD39/ENTPD1 Polyclonal antibody

CD39/ENTPD1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14211-1-AP

Synonyms

ATPDase, CD39, CD39/ENTPD1, Ecto apyrase, Ecto ATP diphosphohydrolase 1, Ecto ATPase 1, Ecto ATPDase 1, ENTPD1, e-NTPDase, NTPDase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse placenta tissue using CD39/ENTPD1 Polyclonal antibody (14211-1-AP)

    WB analysis of mouse placenta using 14211-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 14211-1-AP (ENTPD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using CD39/ENTPD1 Polyclonal antibody (14211-1-AP)

    WB analysis of human placenta using 14211-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CD39/ENTPD1 Polyclonal antibody (14211-1-AP)

    WB analysis using 14211-1-AP

    human placenta lysates were subjected to SDS PAGE followed by western blot with 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD39/ENTPD1 Polyclonal antibody (14211-1-AP)

    IHC staining of human placenta using 14211-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD39/ENTPD1 Polyclonal antibody (14211-1-AP)

    IHC staining of human tonsillitis using 14211-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD39/ENTPD1 Polyclonal antibody (14211-1-AP)

    IHC staining of human placenta using 14211-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD39/ENTPD1 Polyclonal antibody (14211-1-AP)

    IHC staining of human placenta using 14211-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD39/ENTPD1 Polyclonal antibody (14211-1-AP)

    IHC staining of human tonsillitis using 14211-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD39/ENTPD1 Polyclonal antibody (14211-1-AP)

    IHC staining of human tonsillitis using 14211-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD39/ENTPD1 Polyclonal antibody (14211-1-AP)

    IHC staining of human placenta using 14211-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using CD39/ENTPD1 Polyclonal antibody (14211-1-AP)

    IHC staining of human liver using 14211-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using CD39/ENTPD1 Polyclonal antibody (14211-1-AP)

    IHC staining of human liver using 14211-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD39/ENTPD1 Polyclonal antibody (14211-1-AP)

    IF Staining of HeLa using 14211-1-AP

    Immunofluorescent analysis of HeLa cells using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD39/ENTPD1 Polyclonal antibody (14211-1-AP)

    IF Staining of HeLa using 14211-1-AP

    Immunofluorescent analysis of HeLa cells using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of Jurkat cells using CD39/ENTPD1 Polyclonal antibody (14211-1-AP)

    FC experiment of Jurkat using 14211-1-AP

    1X10^6 Jurkat cells were stained with 0.5ug ENTPD1 antibody (14211-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inmouse placenta tissue, human placenta tissue
Positive IHC detected inhuman placenta tissue, human tonsillitis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14211-1-AP targets CD39/ENTPD1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD39/ENTPD1 fusion protein Ag5429
Full Name ectonucleoside triphosphate diphosphohydrolase 1
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 50 kDa，70-100 kDa
GenBank Accession NumberBC047664
Gene Symbol ENTPD1
Gene ID (NCBI) 953
RRIDAB_10638609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named as CD39, NTPDase 1, Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.It is a noncovalent tetrameric protein and that detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ).And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa and 34 kDa) produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CD39/ENTPD1 antibody 14211-1-APDownload protocol
IHC protocol for CD39/ENTPD1 antibody 14211-1-APDownload protocol
IF protocol for CD39/ENTPD1 antibody 14211-1-APDownload protocol
FC protocol for CD39/ENTPD1 antibody 14211-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Int J Biol Sci

CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence

Authors - Zhenni Liu
View Article
humanIF

Genomics Proteomics Bioinformatics

Single-cell Immune Landscape of Human Recurrent Miscarriage.

Authors - Feiyang Wang
View Article
humanIHC

Oncotarget

Expression of CD38 in myeloma bone niche: A rational basis for the use of anti-CD38 immunotherapy to inhibit osteoclast formation.

Authors - Federica Costa
View Article
ratIHC

Am J Physiol Gastrointest Liver Physiol

Water intake accelerates ATP release from myofibroblast cells in rats: ATP-mediated podoplanin-dependent control for physiological function and immunity.

Authors - Moyuru Hayashi
View Article
humanWB

BMC Med Genomics

Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis.

Authors - Xing-Cheng Zhao
View Article
ratWB

J Gerontol A Biol Sci Med Sci

Hypoxanthine Induces Signs of Bladder Aging with Voiding dysfunction and Lower Urinary Tract Remodeling

Authors - Lori A Birder
View Article