CD39/ENTPD1 Polyclonal antibody

Name: CD39/ENTPD1 Polyclonal antibody
Brand: Proteintech
SKU: 19229-1-AP
Rating: 5.00 (1 reviews)

CD39/ENTPD1 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 19229-1-AP

Synonyms

ATPDase, CD39, CD39/ENTPD1, Ecto apyrase, Ecto ATP diphosphohydrolase 1, Ecto ATPase 1, Ecto ATPDase 1, ENTPD1, e-NTPDase, NTPDase 1

Validation Data Gallery

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AllWBIPIHCIFFC

WB analysis of mouse liver using 19229-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 19229-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 19229-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 19229-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19229-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19229-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19229-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19229-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19229-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 19229-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 19229-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 19229-1-AP
IP result of anti-CD39/ENTPD1 (IP:19229-1-AP, 4ug; Detection:19229-1-AP 1:800) with mouse liver tissue lysate 4000ug.
IHC staining of human placenta using 19229-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 19229-1-AP (CD39 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 19229-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 19229-1-AP (CD39 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 19229-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 19229-1-AP (CD39 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 19229-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 19229-1-AP (CD39 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver using 19229-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 19229-1-AP (CD39 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 19229-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 19229-1-AP (CD39 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human tonsillitis using 19229-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis using 19229-1-AP (CD39 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human tonsillitis using 19229-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis using 19229-1-AP (CD39 antibody) at dilution of 1:200 (under 40x lens).
IF Staining of human placenta using 19229-1-AP
Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD39/ENTPD1 antibody (19229-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human placenta using 19229-1-AP
Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD39/ENTPD1 antibody (19229-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of Jurkat using 19229-1-AP
1X10^6 Jurkat cells were stained with 0.5ug CD39 antibody (19229-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Cat No. 19229-1-AP

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Tested Applications

Positive WB detected in	mouse liver tissue, HL-60 cells, mouse brain tissue, human liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human placenta tissue, human liver tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	human placenta tissue
Positive FC detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:50-1:500
Flow Cytometry (FC)	FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IHC	See 2 publications below
IF	See 1 publications below
FC	See 1 publications below

Product Information

19229-1-AP targets CD39/ENTPD1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CD39/ENTPD1 fusion protein Ag6165
Full Name	ectonucleoside triphosphate diphosphohydrolase 1
Calculated Molecular Weight	58 kDa
Observed Molecular Weight	75-95 kDa
GenBank Accession Number	BC047664
Gene Symbol	ENTPD1
Gene ID (NCBI)	953
RRID	AB_10859825
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). It has 3 isoforms produced by alternative splicing. CD39 preferentially occurred as a dimer that could be dissociated into monomeric forms. (PMID: 12694193). The soluble isoform of CD39(38-478 amino acid) is located in the extracellular part and 19229-1-AP recognizes both the 3 isoforms and the soluble form of CD39 (38-478aa) molecule.

Protocols

Product Specific Protocols
WB protocol for CD39/ENTPD1 antibody 19229-1-AP	Download protocol
IHC protocol for CD39/ENTPD1 antibody 19229-1-AP	Download protocol
IF protocol for CD39/ENTPD1 antibody 19229-1-AP	Download protocol
IP protocol for CD39/ENTPD1 antibody 19229-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Bone Res Alpl prevents bone ageing sensitivity by specifically regulating senescence and differentiation in mesenchymal stem cells. Authors - Wenjia Liu View Article
human	WB	Biochim Biophys Acta Mol Basis Dis Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages Authors - Ling Zhou View Article
human	FC	J Chem Inf Model Identification of Novel CD39 Inhibitors Based on Virtual Screening and Enzymatic Assays. Authors - Yunshuo Zhao View Article
rat	WB	Biochem J Characterization of soluble CD39 (SolCD39/NTPDase1) from PiggyBac nonviral system as a tool to control the nucleotides level. Authors - Liziane Raquel Beckenkamp View Article
mouse	IHC	JCI Insight Epithelial microRNA-206 targets CD39/extracellular ATP to upregulate airway IL-25 and TSLP in type 2-high asthma. Authors - Kan Zhang View Article
mouse	WB,IHC	Cancer Immunol Immunother STAT3 promotes differentiation of monocytes to MDSCs via CD39/CD73-adenosine signal pathway in oral squamous cell carcinoma Authors - Hao Cui View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Isabelle (Verified Customer) (10-08-2020)

good antibody for western blot

Applications: Western Blot
Primary Antibody Dilution: 1/500
Cell Tissue Type: human liver, hepG2 cell line, renal tubular HK2 cell line

CD39/ENTPD1 Polyclonal antibody

CD39/ENTPD1 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Validation Data Gallery

WB analysis of mouse liver using 19229-1-AP

WB analysis of mouse liver using 19229-1-AP

WB analysis of mouse liver using 19229-1-AP

WB analysis of mouse liver using 19229-1-AP

WB analysis of mouse brain using 19229-1-AP

WB analysis of mouse brain using 19229-1-AP

WB analysis of mouse brain using 19229-1-AP

WB analysis of mouse brain using 19229-1-AP

WB analysis of mouse brain using 19229-1-AP

WB analysis of human liver using 19229-1-AP

WB analysis of HL-60 using 19229-1-AP

IP experiment of mouse liver using 19229-1-AP

IHC staining of human placenta using 19229-1-AP

IHC staining of human placenta using 19229-1-AP

IHC staining of human placenta using 19229-1-AP

IHC staining of human placenta using 19229-1-AP

IHC staining of human liver using 19229-1-AP

IHC staining of human liver using 19229-1-AP

IHC staining of human tonsillitis using 19229-1-AP

IHC staining of human tonsillitis using 19229-1-AP

IF Staining of human placenta using 19229-1-AP

IF Staining of human placenta using 19229-1-AP

FC experiment of Jurkat using 19229-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Alpl prevents bone ageing sensitivity by specifically regulating senescence and differentiation in mesenchymal stem cells.

Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages

Identification of Novel CD39 Inhibitors Based on Virtual Screening and Enzymatic Assays.

Characterization of soluble CD39 (SolCD39/NTPDase1) from PiggyBac nonviral system as a tool to control the nucleotides level.

Epithelial microRNA-206 targets CD39/extracellular ATP to upregulate airway IL-25 and TSLP in type 2-high asthma.

STAT3 promotes differentiation of monocytes to MDSCs via CD39/CD73-adenosine signal pathway in oral squamous cell carcinoma

Reviews