|Positive WB detected in||HeLa cells, HepG2 cells, MCF-7 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells|
|Positive IHC detected in||human ovary tumor tissue, human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:6000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
66192-1-Ig targets ERK1/2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||ERK1/2 fusion protein Ag20350|
|Full Name||mitogen-activated protein kinase 3|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||38-44 kDa|
|GenBank accession number||BC013992|
|Gene ID (NCBI)||5595|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to insulin and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
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