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ERK1/2 Antibody 5 Publications

Mouse Monoclonal| Catalog number: 66192-1-Ig |CloneNo.: 4G8A11

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Con: 186 μg/150 μl

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Tested Applications
Applications:
ELISA, IF, IHC, IP, WB
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Images See all

66192-1-Ig;HeLa cells

HeLa cells were subjected to SDS PAGE followed by western blot with 66192-1-Ig( ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

66192-1-Ig;HeLa cells

IP Result of anti-ERK1/2 (IP:66192-1-Ig, 5ug; Detection:66192-1-Ig 1:2000) with HeLa cells lysate 2960ug.

66192-1-Ig;human ovary tumor tissue

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig( ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0

66192-1-Ig;HepG2 cells

Immunofluorescent analysis of HepG2 cells using 66192-1-Ig(ERK1/2 antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, mouse
Positive WB detected in:
HeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human ovary tumor tissue, human cervical cancer tissue, human ovary tumor tissue
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
IHC : 1:100-1:400
IF : 1:20-1:200

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ERK1/2 fusion protein ag20350
Full name:
mitogen-activated protein kinase 3
Calculated molecular weight:
44 kDa
Observed molecular weight:
38 kDa,43 kDa
GenBank accession number:
BC013992
Gene ID (NCBI):
5595
Gene symbol
MAPK3
Synonyms
ERK, ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3
Background

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to insulin and NGF.


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