ERK1/2 Monoclonal antibody
ERK1/2 Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, CoIP,ELISA
Conjugate
Unconjugated
CloneNo.
4G8A11
Cat no : 66192-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human ovary tumor tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:6000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 16 publications below |
| CoIP | See 1 publications below |
Product Information
66192-1-Ig targets ERK1/2 in WB, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ERK1/2 fusion protein Ag20350 |
| Full Name | mitogen-activated protein kinase 3 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 38-44 kDa |
| GenBank accession number | BC013992 |
| Gene symbol | MAPK3 |
| Gene ID (NCBI) | 5595 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to insulin and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
Publications
| Species | Application | Title |
|---|---|---|
Eur J Pharmacol Triptolide prevents proliferation and migration of Esophageal Squamous Cell Cancer via MAPK/ERK signaling pathway. | ||
Mol Ther Nucleic Acids lncRNA NR_038323 Suppresses Renal Fibrosis in Diabetic Nephropathy by Targeting the miR-324-3p/DUSP1 Axis. | ||
PLoS Biol mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice. | ||
Mol Med Rep Formononetin inhibits colon carcinoma cell growth and invasion by microRNA‑149‑mediated EphB3 downregulation and inhibition of PI3K/AKT and STAT3 signaling pathways. | ||
Exp Ther Med Proof-of-concept study investigating the role of S100P-RAGE in nasopharyngeal carcinoma. | ||
