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ERK1/2 Antibody 9 Publications

Mouse Monoclonal| Catalog number: 66192-1-Ig |CloneNo.: 4G8A11

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Con: 186 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

ERK1/2 Antibody WB HeLa cells 66192-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours

ERK1/2 Antibody IHC human ovary tumor tissue 66192-1-Ig

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:4000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ERK1/2 Antibody IHC human ovary tumor tissue 66192-1-Ig

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:4000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ERK1/2 Antibody IF HepG2 cells 66192-1-Ig

Immunofluorescent analysis of HepG2 cells using 66192-1-Ig(ERK1/2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, mouse
Positive WB detected in:
HeLa cells, HepG2 cells, MCF-7 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected in:
human ovary tumor tissue, human cervical cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:2000-1:10000
IHC : 1:2000-1:6000
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ERK1/2 fusion protein Ag20350
Full name:
mitogen-activated protein kinase 3
Calculated molecular weight:
44 kDa
Observed molecular weight:
38 kDa,43 kDa
GenBank accession number:
BC013992
Gene ID (NCBI):
5595
Gene symbol
MAPK3
Synonyms
ERK, ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3

Background

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to insulin and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-43 kDa.


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