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  • KD/KO Validated

ERO1LB Antibody

ERO1LB Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11261-2-AP

Synonyms

ERO1 L beta, ERO1 like beta (S. cerevisiae), ERO1 like protein beta, ERO1LB, Oxidoreductin 1 L beta



Tested Applications

Positive WB detected inHeLa cells, human placenta tissue
Positive IHC detected inhuman pancreas cancer tissue, human oesophagus tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11261-2-AP targets ERO1LB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERO1LB fusion protein Ag1776
Full Name ERO1-like beta (S. cerevisiae)
Calculated molecular weight 54 kDa
Observed molecular weight 40~50 kDa
GenBank accession number BC032823
Gene symbol ERO1LB
Gene ID (NCBI) 56605
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Endoplasmic reticulum oxidoreductases (Eros) are essential for the formation of disulfide bonds. Mammals express two related Ero proteins, Ero1L alpha and Ero1L beta. The two homologous proteins can form homodimers and mixed heterodimers. ERO1-Lα and -β are highly expressed in organs with a high demand for secretion, consistent with their roles in oxidative folding of ER proteins.

Protocols

Product Specific Protocols
WB protocol for ERO1LB antibody 11261-2-APDownload protocol
IHC protocol for ERO1LB antibody 11261-2-APDownload protocol
IF protocol for ERO1LB antibody 11261-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Toxicol

Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage Alcoholic Liver Disease.

Authors - Galligan James J JJ
humanWB

Sci Rep

Hypoxia-inducible ERO1α promotes cancer progression through modulation of integrin-β1 modification and signalling in HCT116 colorectal cancer cells.

Authors - Norio Takei
  • KO Validated
humanWB

J Biol Chem

Action of protein disulfide isomerase on proinsulin exit from endoplasmic reticulum of pancreatic β-cells.

Authors - Rajpal Gautam G
mouseWB

Endocrinology

Endoplasmic reticulum oxidoreductin-1-like β (ERO1lβ) regulates susceptibility to endoplasmic reticulum stress and is induced by insulin flux in β-cells.

Authors - Khoo Cynthia C
  • KD Validated
mouseWB

Hum Mol Genet

Curcumin Facilitates a Transitory Cellular Stress Response in Trembler-J Mice.

Authors - Okamoto Yuji Y