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  • Featured Product
  • KD/KO Validated

EXOSC10 Polyclonal antibody

EXOSC10 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16731-1-AP

Synonyms

Autoantigen PM/Scl 2, EXOSC10, exosome component 10, p2, p3, p4, PM Scl, PM/Scl 100, PMSCL, PMSCL2, RRP6, Rrp6p

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using EXOSC10 Polyclonal antibody (16731-1-AP)

    WB analysis of HeLa using 16731-1-AP

    WB result of EXOSC10 antibody (16731-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC10 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using EXOSC10 Polyclonal antibody (16731-1-AP)

    WB analysis using 16731-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16731-1-AP (EXOSC10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using EXOSC10 Polyclonal antibody (16731-1-AP)

    WB analysis of mouse spleen using 16731-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16731-1-AP (EXOSC10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using EXOSC10 Polyclonal antibody (16731-1-AP)

    IP experiment of HeLa using 16731-1-AP

    IP result of anti-EXOSC10 (IP:16731-1-AP, 4ug; Detection:16731-1-AP 1:700) with HeLa cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using EXOSC10 Polyclonal antibody (16731-1-AP)

    IHC staining of human stomach cancer using 16731-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16731-1-AP (EXOSC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using EXOSC10 Polyclonal antibody (16731-1-AP)

    IHC staining of human stomach cancer using 16731-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16731-1-AP (EXOSC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using EXOSC10 Polyclonal antibody (16731-1-AP)

    IHC staining of human liver cancer using 16731-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16731-1-AP (EXOSC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EXOSC10 Polyclonal antibody (16731-1-AP)

    IF Staining of HeLa using 16731-1-AP

    Immunofluorescent analysis of HeLa cells using 16731-1-AP (EXOSC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using EXOSC10 Polyclonal antibody (16731-1-AP)

    IF Staining of NIH/3T3 using 16731-1-AP

    Immunofluorescent analysis of NIH/3T3 cells using 16731-1-AP (EXOSC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, mouse spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16731-1-AP targets EXOSC10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOSC10 fusion protein Ag10189
Full Name exosome component 10
Calculated Molecular Weight 885 aa, 101 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC073788
Gene Symbol EXOSC10
Gene ID (NCBI) 5394
RRIDAB_2101730
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

Protocols

Product Specific Protocols
WB protocol for EXOSC10 antibody 16731-1-APDownload protocol
IHC protocol for EXOSC10 antibody 16731-1-APDownload protocol
IF protocol for EXOSC10 antibody 16731-1-APDownload protocol
IP protocol for EXOSC10 antibody 16731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

PeerJ

EXOSC10 is a novel hepatocellular carcinoma prognostic biomarker: a comprehensive bioinformatics analysis and experiment verification

Authors - Zhi-Yong Meng
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tsimafei (Verified Customer) (12-17-2023)

Antibody recognise Exosc10 band and some other non-specific bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mESCs
EXOSC10 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:16731-1-AP)