EXOSC2 Monoclonal antibody

EXOSC2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G8B1

Cat no : 66099-1-Ig

Synonyms

EXOSC2, exosome component 2, hRrp4p, p7, RRP4, Rrp4p



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman liver tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66099-1-Ig is EXOSC2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EXOSC2 fusion protein Ag7003
Full Name exosome component 2
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC000747
Gene symbol EXOSC2
Gene ID (NCBI) 23404
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snoRNA and snRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs [PMID:15346807]. EXOSC2 is a non-catalytic component of the RNA exosome complex that has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events [PMID: 17545563].

Protocols

Product Specific Protocols
WB protocol for EXOSC2 antibody 66099-1-IgDownload protocol
IHC protocol for EXOSC2 antibody 66099-1-IgDownload protocol
IF protocol for EXOSC2 antibody 66099-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Cell

The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.

Authors - Jakob Trendel