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EXOSC2 Antibody 1 Publications

Mouse Monoclonal| Catalog number: 66099-1-Ig |CloneNo.: 1G8B1

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Con: 190 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

EXOSC2 Antibody WB Jurkat cells 66099-1-Ig

Jurkat cells were subjected to SDS PAGE followed by western blot with 66099-1-Ig(EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

EXOSC2 Antibody IHC human skin cancer tissue 66099-1-Ig

Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66099-1-Ig( EXOSC2 Antibody) at dilution of 1:50

EXOSC2 Antibody IHC human liver tissue 66099-1-Ig

Immunohistochemical analysis of paraffin-embedded human liver slide using 66099-1-Ig( EXOSC2 Antibody) at dilution of 1:50

EXOSC2 Antibody IF HepG2 cells 66099-1-Ig

Immunofluorescent analysis of ( ) fixed HepG2 cells using 66099-1-Ig (EXOSC2 antibody) at dilution of 1:25 and N/A

Species specificity:
human
Positive WB detected in:
Jurkat cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected in:
human liver tissue, human skin cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2a
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
EXOSC2 fusion protein Ag7003
Full name:
exosome component 2
Calculated molecular weight:
33 kDa
Observed molecular weight:
33 kDa
GenBank accession number:
BC000747
Gene ID (NCBI):
23404
Gene symbol
EXOSC2
Synonyms
EXOSC2, exosome component 2, hRrp4p, p7, RRP4, Rrp4p

Background

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snoRNA and snRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs [PMID:15346807]. EXOSC2 is a non-catalytic component of the RNA exosome complex that has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events [PMID: 17545563].


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