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  • KD/KO Validated

FASTKD2 Polyclonal antibody

FASTKD2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 17464-1-AP

Synonyms

FASTKD 2, FAST kinase domain-containing protein 2, mitochondrial



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inCOS7 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17464-1-AP targets FASTKD2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FASTKD2 fusion protein Ag11314
Full Name FAST kinase domains 2
Calculated Molecular Weight 710 aa, 81 kDa
Observed Molecular Weight 55-65 kDa
GenBank Accession NumberBC001544
Gene Symbol FASTKD2
Gene ID (NCBI) 22868
RRIDAB_2101119
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715)

Protocols

Product Specific Protocols
WB protocol for FASTKD2 antibody 17464-1-APDownload protocol
IF protocol for FASTKD2 antibody 17464-1-APDownload protocol
IP protocol for FASTKD2 antibody 17464-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF,WB

Nat Cell Biol

Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.

Authors - Timo Rey
humanWB

Cell Metab

A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.

Authors - Jason D Arroyo
humanIF

Nucleic Acids Res

FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain.

Authors - Erik Boehm
humanWB

EMBO Rep

A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.

Authors - Hana Antonicka
humanWB, IF, RIP

Cell Rep

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.

Authors - Hana Antonicka
  • KD Validated
IF

Cell Rep

A mitochondria-specific isoform of FASTK is present in mitochondrial RNA granules and regulates gene expression and function.

Authors - Alexis A Jourdain

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Erik (Verified Customer) (02-25-2021)

We used the antibody at 1:500 dilution, and it produced two clean bands for the two protein isoforms annotated in UniProt, at the expected height

  • Applications: Western Blot
  • Cell Tissue Type: PANC1 and MIAPACA2 cell lines
FASTKD2 Antibody Western Blot validation ( dilution) in PANC1 and MIAPACA2 cell lines (Cat no:17464-1-AP)