|Positive WB detected in||HeLa cells, HepG2 cells, K-562 cells|
|Positive IP detected in||mouse heart tissue|
|Positive IF detected in||COS7 cells, U2OS cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17464-1-AP targets FASTKD2 in WB, RIP, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FASTKD2 fusion protein Ag11314|
|Full Name||FAST kinase domains 2|
|Calculated molecular weight||710 aa, 81 kDa|
|Observed molecular weight||55-65 kDa|
|GenBank accession number||BC001544|
|Gene ID (NCBI)||22868|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715)
Methods Mol Biol
Sucrose Gradient Sedimentation Analysis of Mitochondrial Ribosomes.
J Cell Biochem
FASTKD2 promotes cancer cell progression through upregulating Myc expression in pancreatic ductal adenocarcinoma.
J Biol Chem
Role of FAST kinase domains 3 (FASTKD3) in post-transcriptional regulation of mitochondrial gene expression.
J Biol Chem
The Pseudouridine Synthase RPUSD4 is an essential component of Mitochondrial RNA Granules.
Mutations in FASTKD2 are associated with mitochondrial disease with multi-OXPHOS deficiency.
J Cell Sci
Mitochondrial Alkbh1 localises to mtRNA granules and its knockdown induces mitochondrial UPR in humans and C. elegans.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Erik (Verified Customer) (02-25-2021)
We used the antibody at 1:500 dilution, and it produced two clean bands for the two protein isoforms annotated in UniProt, at the expected height