• Featured Product
  • KD/KO Validated

FASTKD2 Polyclonal antibody

FASTKD2 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, RIP, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17464-1-AP

Synonyms

FAST kinase domains 2, FASTKD2, KIAA0971



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells
Positive IP detected inmouse heart tissue
Positive IF detected inCOS7 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17464-1-AP targets FASTKD2 in WB, RIP, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FASTKD2 fusion protein Ag11314
Full Name FAST kinase domains 2
Calculated molecular weight 710 aa, 81 kDa
Observed molecular weight 55-65 kDa
GenBank accession numberBC001544
Gene symbol FASTKD2
Gene ID (NCBI) 22868
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715)

Protocols

Product Specific Protocols
WB protocol for FASTKD2 antibody 17464-1-APDownload protocol
IF protocol for FASTKD2 antibody 17464-1-APDownload protocol
IP protocol for FASTKD2 antibody 17464-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Methods Mol Biol

Sucrose Gradient Sedimentation Analysis of Mitochondrial Ribosomes.

Authors - Austin Choi
humanWB,IHC

J Cell Biochem

FASTKD2 promotes cancer cell progression through upregulating Myc expression in pancreatic ductal adenocarcinoma.

Authors - Rui Fang
  • KD Validated
humanWB

J Biol Chem

Role of FAST kinase domains 3 (FASTKD3) in post-transcriptional regulation of mitochondrial gene expression.

Authors - Erik Boehm
IF

J Biol Chem

The Pseudouridine Synthase RPUSD4 is an essential component of Mitochondrial RNA Granules.

Authors - Sofia Zaganelli
human,zebrafishWB

Hum Mutat

Mutations in FASTKD2 are associated with mitochondrial disease with multi-OXPHOS deficiency.

Authors - Xiujuan Wei
  • KD Validated

J Cell Sci

Mitochondrial Alkbh1 localises to mtRNA granules and its knockdown induces mitochondrial UPR in humans and C. elegans.

Authors - Anita Wagner

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Erik (Verified Customer) (02-25-2021)

We used the antibody at 1:500 dilution, and it produced two clean bands for the two protein isoforms annotated in UniProt, at the expected height

  • Applications: Western Blot
  • Cell Tissue Type: PANC1 and MIAPACA2 cell lines
FASTKD2 Antibody Western Blot validation ( dilution) in PANC1 and MIAPACA2 cell lines (Cat no:17464-1-AP)