Tested Applications
| Positive WB detected in | mouse colon tissue, human ileum tissue, mouse thymus tissue, rat thymus tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11827-1-AP targets FGL2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2395 Product name: Recombinant human FGL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-148 aa of BC017813 Sequence: MKLANWYWLSSAVLAAYGFLVVANNETEEIKDERAKDVCPVRLESRGKCEEAGECPYQVSLPPLTIQLPKQFSRIEEVFKEVQNLKEIVNSLKKSCQDCKLQADDNGDPGRNGLLLPSTGAPGEVGDNRVRELESEVNKLSSELKFIF Predict reactive species |
| Full Name | fibrinogen-like 2 |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 70 kDa, 50-60 kDa |
| GenBank Accession Number | BC017813 |
| Gene Symbol | FGL2 |
| Gene ID (NCBI) | 10875 |
| RRID | AB_2103947 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14314 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FGL2 (fibrinogen like protein 2) is a 70 kDa glycoprotein that belongs to the fibrinogen-related superfamily of proteins which are involved in coagulation, cell adhesion, and transendothelial migration. FGL2 is an important immune regulator of both innate and adaptive response. It is present on the surface of macrophages and endothelial cells, and can be constitutively secreted by CD4+ CD8+ T cell. FGL2 forms a tetrameric complex which is stabilized by interchain disulfide bonds and it exerts immunosuppressive effects on both T cell proliferation and dendritic cell maturation. The protein may play a role in physiologic functions at mucosal sites.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FGL2 antibody 11827-1-AP | Download protocol |
| IHC protocol for FGL2 antibody 11827-1-AP | Download protocol |
| WB protocol for FGL2 antibody 11827-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-FGL2 (Fibrinogen-like protein 2) antibody, catalog number 11827-1-AP, has 17 total citations published across 15 journals, including Nature Communications, Cell Metabolism, Frontiers in Immunology, International Immunopharmacology, Oncoimmunology, Molecular Immunology, Phytomedicine, and Heliyon. Associated research fields span immunology and inflammation, neuroscience, cardiology, hepatology, oncology, nephrology, ophthalmology, and diabetes.
| Species | Application | Title |
|---|---|---|
Cell Metab Bone marrow immune cells respond to fluctuating nutritional stress to constrain weight regain | ||
Nat Commun Cerebrospinal fluid lipocalin 2 as a novel biomarker for the differential diagnosis of vascular dementia. | ||
Carbohydr Polym Antibacterial chitosan/organic rectorite nanocomposite-conjugated gelatin/β-cyclodextrin hydrogels with improved hemostasis performance for wound repair | ||
Cell Commun Signal Single-cell profiling reveals periosteal signatures of impaired periosteal cells proliferation in a drill-hole model of type 2 diabetes. | ||
Phytomedicine Cuyun Recipe ameliorates pregnancy loss by regulating macrophage polarization and hypercoagulable state during the peri-implantation period in an ovarian hyperstimulation mouse model | ||
Front Immunol Clara Cell 10 kDa Protein Alleviates Murine Hepatitis Virus Strain 3-Induced Fulminant Hepatitis by Inhibiting Fibrinogen-Like Protein 2 Expression. |
