|Positive WB detected in||HeLa cells, mouse heart tissue, pig brain tissue, SH-SY5Y cells, mouse brain tissue, rat brain tissue, rat heart tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||rat brain tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hepa1-6 cells, HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:200|
|Sample-dependent, check data in validation data gallery|
10956-1-AP targets FIS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||cow, hamster, human, mouse, rat, squirrels, swine|
|Host / Isotype||Rabbit / IgG1|
|Immunogen||FIS1 fusion protein Ag1409|
|Full Name||fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)|
|Calculated molecular weight||17 kDa|
|Observed molecular weight||17 kDa|
|GenBank accession number||BC009428|
|Gene ID (NCBI)||51024|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. This antibody was raised against the full-length of human Fis1 protein, and recognizes endogenous Fis1 protein in various lysates. (PMID: 21257639, 21459773, 23333625)
|Product Specific Protocols|
|WB protocol for FIS1 antibody 10956-1-AP||Download protocol|
|IHC protocol for FIS1 antibody 10956-1-AP||Download protocol|
|IF protocol for FIS1 antibody 10956-1-AP||Download protocol|
|IP protocol for FIS1 antibody 10956-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Siting (Verified Customer) (01-28-2020)
This antibody worked perfectly for western blot.
Chun (Verified Customer) (07-03-2019)
This antibody is excellent/
Kishor (Verified Customer) (12-13-2018)
I got good results in both rat liver protein and H69 cells.