Filter: AllWBIPIHCIF/ICC

WB analysis using 10956-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa cells using 10956-1-AP
WB result of FIS1 antibody (10956-1-AP, 1:8,000) with si-Control and si-FIS1 transfected HeLa cells.
WB analysis using 10956-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 10956-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of pig brain using 10956-1-AP
pig brain tissue were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 10956-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10956-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 10956-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 10956-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 10956-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10956-1-AP
IP result of anti-FIS1 (IP:10956-1-AP, 4ug; Detection:10956-1-AP 1:10000) with HeLa cells lysate 1120 ug.
IHC staining of rat brain using 10956-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10956-1-AP (FIS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 10956-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 10956-1-AP (FIS1 antibody) at dilution of 1:25 (under 40x lens).
IF Staining of Hepa1-6 cells using 10956-1-AP
IF result of anti-FIS1(10956-1-AP,1:100) with Hepa1-6 cell by Dr. Steven Eugene Smith. Mitochondrion outer membrane (Green) Stain.
IF Staining of HepG2 using 10956-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10956-1-AP (FIS1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 10956-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FIS1 antibody (10956-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Featured Product
KD/KO Validated

FIS1 Polyclonal antibody

Name: FIS1 Polyclonal antibody
Brand: Proteintech
SKU: 10956-1-AP
Rating: 4.75 (4 reviews)

FIS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10956-1-AP

Publications(352)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (6)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

TTC11, TPR repeat protein 11, hFis1, FIS1 homolog, CGI-135

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (352)
Protocols
Reviews (4)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse brain tissue, rat brain tissue, mouse heart tissue, SH-SY5Y cells, pig brain tissue, HeLa cells, rat heart tissue, Jurkat cells, mouse spleen tissue, rat spleen tissue, HepG2 cells, PC-12 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	rat brain tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, Hepa1-6 cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:14000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 325 publications below
IHC	See 12 publications below
IF	See 39 publications below
IP	See 6 publications below
CoIP	See 1 publications below

Product Information

10956-1-AP targets FIS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse, rat, pig, monkey, chicken, zebrafish, hamster, goat, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FIS1 fusion protein Ag1409 Predict reactive species
Full Name	fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Calculated Molecular Weight	17 kDa
Observed Molecular Weight	17 kDa
GenBank Accession Number	BC009428
Gene Symbol	FIS1
Gene ID (NCBI)	51024
RRID	AB_2102532
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y3D6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. This antibody was raised against the full-length of human Fis1 protein, and recognizes endogenous Fis1 protein in various lysates. (PMID: 21257639, 21459773, 23333625)

Protocols

Product Specific Protocols
WB protocol for FIS1 antibody 10956-1-AP	Download protocol
IHC protocol for FIS1 antibody 10956-1-AP	Download protocol
IF protocol for FIS1 antibody 10956-1-AP	Download protocol
IP protocol for FIS1 antibody 10956-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature Restoring metabolism of myeloid cells reverses cognitive decline in ageing. Authors - Paras S Minhas View Article
	WB,IF	Nature Distinct fission signatures predict mitochondrial degradation or biogenesis. Authors - Tatjana Kleele KO Validated View Article
human	WB	Nat Immunol RNA viruses promote activation of the NLRP3 inflammasome through a RIP1-RIP3-DRP1 signaling pathway. Authors - Xiaqiong Wang KD Validated View Article
human	WB,IF	Cell Stem Cell AMPK/FIS1-Mediated Mitophagy Is Required for Self-Renewal of Human AML Stem Cells. Authors - Shanshan Pei KD Validated View Article
human	WB	Cell Metab Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae. Authors - Jing Yuan View Article
mouse	WB,IP	Nat Metab Mitochondrial fission drives neuronal metabolic burden to promote stress susceptibility in male mice Authors - Wan-Ting Dong View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Megan (Verified Customer) (06-22-2021)	RPE cell over-expressing pcDNA hFis1. Note 1:500 was used here to detect over-expressed Fis1, but we also use 1:100 when detecting endogenous Fis1. Also successfully used to detect endogenous Fis1 levels via Western Blot (used @ 1:1000). Applications: Western Blot, Immunofluorescence Primary Antibody Dilution: 1:100 - 1:500 Cell Tissue Type: RPE FIS1 Antibody Western Blot,Immunofluorescence validation (1:100 - 1:500 dilution) in RPE (Cat no:10956-1-AP)
FH Siting (Verified Customer) (01-28-2020)	This antibody worked perfectly for western blot. Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: Mouse heart tissue
FH Chun (Verified Customer) (07-03-2019)	This antibody is excellent/ Applications: Western Blot, Primary Antibody Dilution: 1:2,000 Cell Tissue Type: HEK293 and HeLa cells, and primary rat cortical neurons
FH Kishor (Verified Customer) (12-13-2018)	I got good results in both rat liver protein and H69 cells. Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: H69, HUCCT-1 FIS1 Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:10956-1-AP)