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  • Featured Product
  • KD/KO Validated

FIS1 Polyclonal antibody

FIS1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (6)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10956-1-AP

Synonyms

CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FIS1 Polyclonal antibody (10956-1-AP)

    WB analysis using 10956-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FIS1 Polyclonal antibody (10956-1-AP)

    WB analysis of HeLa cells using 10956-1-AP

    WB result of FIS1 antibody (10956-1-AP, 1:8,000) with si-Control and si-FIS1 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using FIS1 Polyclonal antibody (10956-1-AP)

    WB analysis using 10956-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FIS1 Polyclonal antibody (10956-1-AP)

    WB analysis using 10956-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using FIS1 Polyclonal antibody (10956-1-AP)

    WB analysis of SH-SY5Y using 10956-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using FIS1 Polyclonal antibody (10956-1-AP)

    WB analysis of rat brain using 10956-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using FIS1 Polyclonal antibody (10956-1-AP)

    WB analysis of mouse brain using 10956-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using FIS1 Polyclonal antibody (10956-1-AP)

    WB analysis of pig brain using 10956-1-AP

    pig brain tissue were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using FIS1 Polyclonal antibody (10956-1-AP)

    WB analysis of mouse heart using 10956-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using FIS1 Polyclonal antibody (10956-1-AP)

    WB analysis of rat heart using 10956-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 10956-1-AP (FIS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using FIS1 Polyclonal antibody (10956-1-AP)

    IP experiment of HeLa using 10956-1-AP

    IP Result of anti-FIS1 (IP:10956-1-AP, 3ug; Detection:10956-1-AP 1:500) with HeLa cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of rat brain tissue using FIS1 Polyclonal antibody (10956-1-AP)

    IHC staining of rat brain using 10956-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10956-1-AP (FIS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using FIS1 Polyclonal antibody (10956-1-AP)

    IHC staining of human brain using 10956-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 10956-1-AP (FIS1 antibody) at dilution of 1:25 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of Hepa1-6 cells using FIS1 Polyclonal antibody (10956-1-AP)

    IF Staining of Hepa1-6 cells using 10956-1-AP

    IF result of anti-FIS1(10956-1-AP,1:100) with Hepa1-6 cell by Dr. Steven Eugene Smith. Mitochondrion outer membrane (Green) Stain.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FIS1 Polyclonal antibody (10956-1-AP)

    IF Staining of HepG2 using 10956-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10956-1-AP (FIS1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, rat brain tissue, mouse heart tissue, pig brain tissue, SH-SY5Y cells, rat heart tissue, mouse brain tissue, HepG2 cells, Jurkat cells, mouse spleen tissue, rat spleen tissue, PC-12 cells
Positive IP detected inHeLa cells
Positive IHC detected inrat brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepa1-6 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

10956-1-AP targets FIS1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, goat, chicken, zebrafish, hamster, pig, squirrel, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FIS1 fusion protein Ag1409
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC009428
Gene symbol FIS1
Gene ID (NCBI) 51024
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. This antibody was raised against the full-length of human Fis1 protein, and recognizes endogenous Fis1 protein in various lysates. (PMID: 21257639, 21459773, 23333625)

Protocols

Product Specific Protocols
WB protocol for FIS1 antibody 10956-1-APDownload protocol
IHC protocol for FIS1 antibody 10956-1-APDownload protocol
IF protocol for FIS1 antibody 10956-1-APDownload protocol
IP protocol for FIS1 antibody 10956-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Restoring metabolism of myeloid cells reverses cognitive decline in ageing.

Authors - Paras S Minhas
View Article
WB,IF

Nature

Distinct fission signatures predict mitochondrial degradation or biogenesis.

Authors - Tatjana Kleele
  • KO Validated
View Article
humanWB

Nat Immunol

RNA viruses promote activation of the NLRP3 inflammasome through a RIP1-RIP3-DRP1 signaling pathway.

Authors - Xiaqiong Wang
  • KD Validated
View Article
humanWB,IF

Cell Stem Cell

AMPK/FIS1-Mediated Mitophagy Is Required for Self-Renewal of Human AML Stem Cells.

Authors - Shanshan Pei
  • KD Validated
View Article
humanWB

Cell Metab

Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae.

Authors - Jing Yuan
View Article
mouseWB

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Megan (Verified Customer) (06-22-2021)

RPE cell over-expressing pcDNA hFis1. Note 1:500 was used here to detect over-expressed Fis1, but we also use 1:100 when detecting endogenous Fis1. Also successfully used to detect endogenous Fis1 levels via Western Blot (used @ 1:1000).

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:100 - 1:500
  • Cell Tissue Type: RPE
FIS1 Antibody Western Blot,Immunofluorescence validation (1:100 - 1:500 dilution) in RPE (Cat no:10956-1-AP)
FH

Siting (Verified Customer) (01-28-2020)

This antibody worked perfectly for western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart tissue
FH

Chun (Verified Customer) (07-03-2019)

This antibody is excellent/

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2,000
  • Cell Tissue Type: HEK293 and HeLa cells, and primary rat cortical neurons
FH

Kishor (Verified Customer) (12-13-2018)

I got good results in both rat liver protein and H69 cells.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1
FIS1 Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:10956-1-AP)