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  • KD/KO Validated

Fascin Polyclonal antibody

Fascin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14384-1-AP

Synonyms

55 kDa actin bundling protein, FAN1, Fascin, Fascin 1,Fascin, FSCN1, HSN, p55, Singed like protein, SNL



Tested Applications

Positive WB detected inHeLa cells, mouse thymus tissue, K-562 cells, NIH/3T3 cells, SH-SY5Y cells
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14384-1-AP targets Fascin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Fascin fusion protein Ag5816
Full Name fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC000521
Gene Symbol FSCN1
Gene ID (NCBI) 6624
RRIDAB_2106381
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

Protocols

Product Specific Protocols
WB protocol for Fascin antibody 14384-1-APDownload protocol
IHC protocol for Fascin antibody 14384-1-APDownload protocol
IF protocol for Fascin antibody 14384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

Exosomal lncRNA LINC01711 facilitates metastasis of esophageal squamous cell carcinoma via the miR-326/FSCN1 axis.

Authors - Mei-Ling Xu
humanWB

Int J Biol Macromol

Recombinant porcine NK-lysin inhibits the invasion of hepatocellular carcinoma cells in vitro.

Authors - Ajab Khan
humanWB

Exp Cell Res

Downregulation of fascin in the first trimester placental villi is associated with early recurrent miscarriage.

Authors - Jones Lamptey
  • KD Validated
humanWB

J Exp Clin Cancer Res

The role of microRNA-133b and its target gene FSCN1 in gastric cancer.

Authors - Lihua Guo
mouseWB

Immunol Lett

Yes-associated protein upregulates filopodia formation to promote alveolar epithelial-cell phagocytosis.

Authors - Peiyu Gao
  • KD Validated
humanIHC

J Anat

Localization of macrophages and dendritic cells in human thoracic lymph nodes: An immunohistochemical study using surgically obtained specimens

Authors - Masaya Aoki

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ryan (Verified Customer) (01-24-2018)

Antigen retrieval NaCit 0.1M ph6

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Retina/Brain
Fascin Antibody Immunohistochemistry, validation (1:100 dilution) in Retina/Brain (Cat no:14384-1-AP)