FXYD2 Polyclonal antibody

FXYD2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11198-1-AP

Synonyms

ATP1C, ATP1G1, FXYD2, HOMG2, Sodium pump gamma chain



Tested Applications

Positive WB detected inHEK-293 cells, human liver tissue, human skeletal muscle tissue, mouse skeletal muscle tissue, human kidney tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

11198-1-AP targets FXYD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FXYD2 fusion protein Ag1676
Full Name FXYD domain containing ion transport regulator 2
Calculated molecular weight 7 kDa
Observed molecular weight 7 kDa
GenBank accession numberBC013289
Gene symbol FXYD2
Gene ID (NCBI) 486
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FXYD2 (FXYD domain-containing ion transport regulator 2), also known as the gamma-subunit of the NaK-ATPase, belongs to the FXYD family which has been proposed to be the regulators of Na, K-ATPase function by lowering affinities of the system for potassium and sodium. The expression of FXYD2 is most abundant in kidney, while it is also detected in several other tissues like placenta, pancreas, and dorsal root ganglia (DRGs). Three splice variants of FXYD2 have been reported in mouse kidney, namely FXYD2 γa, -γb,and -γc. FXYD2 γa has been identified as a pancreatic beta cell-specific biomarker. This antibody can recognize all three isoforms of FXYD2.

Protocols

Product Specific Protocols
WB protocol for FXYD2 antibody 11198-1-APDownload protocol
IHC protocol for FXYD2 antibody 11198-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Cell

Identification of stem cell populations in sweat glands and ducts reveals roles in homeostasis and wound repair.

Authors - Lu Catherine P CP