FXYD3 Polyclonal antibody

FXYD3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15853-1-AP

Synonyms

FXYD3, Mammary tumor 8 kDa protein, MAT 8, MAT8, Phospholemman like, PLML



Tested Applications

Positive WB detected inCOLO 320 cells, A375 cells, SGC-7901 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15853-1-AP targets FXYD3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FXYD3 fusion protein Ag8672
Full Name FXYD domain containing ion transport regulator 3
Calculated molecular weight 87 aa, 9 kDa
Observed molecular weight 8 kDa
GenBank accession numberBC005238
Gene symbol FXYD3
Gene ID (NCBI) 5349
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FXYD3 antibody 15853-1-APDownload protocol
IHC protocol for FXYD3 antibody 15853-1-APDownload protocol
IP protocol for FXYD3 antibody 15853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols