Factor XIIIa Monoclonal antibody

Factor XIIIa Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, mouse, rat, rabbit

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2D2B2

Cat no : 66325-1-Ig

Synonyms

Coagulation factor XIIIa, F13A, F13A1, Factor XIIIA, Transglutaminase A chain



Tested Applications

Positive WB detected inpig colon tissue, human placenta tissue, human peripheral blood platelets, rat colon tissue, rabbit large intestine tissue, pig liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inU-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66325-1-Ig targets Factor XIIIa in WB, IHC, FC, ELISA applications and shows reactivity with human, pig, mouse, rat, rabbit samples.

Tested Reactivity human, pig, mouse, rat, rabbit
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Factor XIIIa fusion protein Ag11105
Full Name coagulation factor XIII, A1 polypeptide
Calculated molecular weight 732 aa, 83 kDa
Observed molecular weight 75-80 kDa
GenBank accession numberBC027963
Gene symbol F13A1
Gene ID (NCBI) 2162
RRIDAB_2881706
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Factor XIIIa is a blood proenzyme that catalyzes intermolecular cross-linking of fibrinogen and is involved in clotting cascade. It has been identified in various tissues including placenta, uterus, and prostate. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (90% positive) and desmoplastic malignant melanoma (negative). Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor , and meningioma.

Protocols

Product Specific Protocols
WB protocol for Factor XIIIa antibody 66325-1-IgDownload protocol
IHC protocol for Factor XIIIa antibody 66325-1-IgDownload protocol
FC protocol for Factor XIIIa antibody 66325-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Inflamm Regen

Expression of factor XIII originating from synovial fibroblasts and macrophages induced by interleukin-6 signaling

Authors - Hirofumi Watanabe