"Mouse IgG2b isotype control Antibodies" Comparison
View side-by-side comparison of Mouse IgG2b isotype control antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | |
| Positive IHC detected in | Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66360-3-Ig targets Mouse IgG2b isotype control in WB, IHC, FC, IP, chIP, ELISA applications and shows reactivity with megathura crenulata samples.
| Tested Reactivity | megathura crenulata |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | Mouse IgG2b isotype control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881740 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Mouse IgG2b isotype control antibody 66360-3-Ig | Download protocol |
| IHC protocol for Mouse IgG2b isotype control antibody 66360-3-Ig | Download protocol |
| WB protocol for Mouse IgG2b isotype control antibody 66360-3-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Mouse IgG2b isotype control Monoclonal antibody (11B8C4), catalog number 66360-3-Ig, has 14 citations published in journals including Immunity, Nature Cell Biology, Nature Communications, Nucleic Acids Research, Cell Death & Disease, EMBO Reports, Science Reports, and PLoS ONE. Associated research fields encompass immunology, coronary atherosclerosis, RNA splicing, hepatocellular carcinoma, polycystic ovary syndrome, antimicrobial nanomaterials, neurodegeneration, ER stress proteostasis, virology, glioma, and single-cell kinase profiling.
| Species | Application | Title |
|---|---|---|
Immunity Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity | ||
Nat Cell Biol Single-cell transcriptomics uncovers endothelial progenitor-like remodelling driving human coronary atherosclerosis progression. | ||
Nat Commun Generation of nanobodies from transgenic 'LamaMice' lacking an endogenous immunoglobulin repertoire | ||
Cell Death Dis E2F1 mediated DDX11 transcriptional activation promotes hepatocellular carcinoma progression through PI3K/AKT/mTOR pathway. | ||
Phytomedicine Kuntai capsule ameliorates PCOS by reversing tryptophan metabolic shift and aberrant nuclear AHR activation: a multi-omics investigation. |
