G3BP1 Polyclonal antibody

Antibody works well for western blot in 1:1000 dilution, incubated overnight in 3%BSA/PBS on human lung cancer cell lines.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Prostate cancer cells

working very well in western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000 for western
• Cell Tissue Type: T24 Bladder cancer cell

Good antibody for flow cytometry

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: OPM2

Antibody works well for western blot in 1:1000 dilution, incubated overnight in 3%BSA/PBS on human lung cancer cell lines.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Lung Cancer Patient Derived Cell Lines

• Applications: Immunofluorescence

• Applications: Immunofluorescence
• Cell Tissue Type: SH-SY5Y

Works great on WB (1/1000 - Overnight 4°C) - Very beautiful IF staining in non stressed (diffuse staining) and Sodium Arsenite-mediated Stress induction (Punctate staining corresponding to stress granules) in HeLa cells (1/250 - 1h at RT).

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

Good product.

• Applications: Immunofluorescence

Good and strong signal.

• Applications: Western Blot

Used for IP and WB of GFP-tagged overexpressed human G3BP1 (HEK293 cells). Lysates were subjected to IP using GFP-Trap beads. WB was done using semi-dry transfer. Antibody was used in 4% milk in TBST at 1:1,000 dilution. It could be reused up to 3 times. As the image shows, the antibody can successfully detect both endogenous and OE protein.

• Applications: Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

• Applications: Western Blot

Best G3BP1 antibody I've used for Western, IF and IP

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: 1:1000 for WB. 1:200 for IF
• Cell Tissue Type: A549 and U2OS

I used the antibody for immunofluorescence imaging to label stress granules induced by treating the cells with with 50µM sodium arsenite. Used the antibody at a dilution 1:100 overnight at 4°C. Worked perfectly well, strong and specific signal. I am very satisfied of this antibody and strongly recommend if for immunofluorescence.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HCT116 cells

Very specific and sensitive

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

This antibody is very specific and good quality.

• Applications: Western Blot,
• Cell Tissue Type: hct-116

PANC1 cells fixed in 4% paraformaldehdye. Bright localization to stress granules.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: human pancreatic adenocarcinoma cells

Very bright staining for stress granule on NaAsO2 treated Hela cells. 1:500 should be sufficient for IF staining.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Hela

It worked great

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:400

Our lab has been using this antibody for IP, WB and IF for many years and it always worked well. I highly recommend this antibody, especially for IP for stress granules.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Cell Tissue Type: HeLa; MEF

Upon induction of sodium arsenite stress in neurons G3BP1 positive stress granules formed in 90 minutes.Cells permeabilized with 0.2% triton for 10 minutes

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1: 500 overnight at 4 degrees
• Cell Tissue Type: Primary motor neurons

I used it for ICC and it worked Great.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1;100
• Cell Tissue Type: NIH3T3