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- KD/KO Validated
G3BP1 Polyclonal antibody
G3BP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse and More (1)
Applications
WB, RIP, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
101
Cat no : 13057-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | C6 cells, HeLa cells, Neuro-2a cells, HEK-293 cells, MCF-7 cells, human brain tissue, HepG2 cells, Jurkat cells, mouse kidney tissue, rat kidney tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | sodium arsenite treated HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF) | IF : 1:300-1:1200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 41 publications below |
| IHC | See 5 publications below |
| IF | See 73 publications below |
| IP | See 6 publications below |
| CoIP | See 2 publications below |
| RIP | See 2 publications below |
Product Information
13057-2-AP targets G3BP1 in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | G3BP1 fusion protein Ag3728 |
| Full Name | GTPase activating protein (SH3 domain) binding protein 1 |
| Calculated molecular weight | 466 aa, 52 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC006997 |
| Gene symbol | G3BP1 |
| Gene ID (NCBI) | 10146 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for G3BP1 antibody 13057-2-AP | Download protocol |
| IHC protocol for G3BP1 antibody 13057-2-AP | Download protocol |
| IF protocol for G3BP1 antibody 13057-2-AP | Download protocol |
| IP protocol for G3BP1 antibody 13057-2-AP | Download protocol |
| FC protocol for G3BP1 antibody 13057-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner. | ||
Cell ELAVL4, splicing, and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids. | ||
Cell RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, Using Annexin A11 as a Molecular Tether. | ||
Cell Phase Separation of FUS Is Suppressed by Its Nuclear Import Receptor and Arginine Methylation. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tatyana (Verified Customer) (01-21-2023) | Used for IP and WB of GFP-tagged overexpressed human G3BP1 (HEK293 cells). Lysates were subjected to IP using GFP-Trap beads. WB was done using semi-dry transfer. Antibody was used in 4% milk in TBST at 1:1,000 dilution. It could be reused up to 3 times. As the image shows, the antibody can successfully detect both endogenous and OE protein.
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FH Tobias (Verified Customer) (10-25-2022) |
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FH Peter (Verified Customer) (10-24-2022) | Best G3BP1 antibody I've used for Western, IF and IP
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FH Patryk (Verified Customer) (03-18-2021) | I used the antibody for immunofluorescence imaging to label stress granules induced by treating the cells with with 50µM sodium arsenite. Used the antibody at a dilution 1:100 overnight at 4°C. Worked perfectly well, strong and specific signal. I am very satisfied of this antibody and strongly recommend if for immunofluorescence.
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FH Kun (Verified Customer) (03-23-2020) | Very specific and sensitive
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FH Biao (Verified Customer) (03-11-2020) | This antibody is very specific and good quality.
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FH Joshua (Verified Customer) (12-28-2019) | PANC1 cells fixed in 4% paraformaldehdye. Bright localization to stress granules.
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FH Yuan (Verified Customer) (11-02-2019) | Very bright staining for stress granule on NaAsO2 treated Hela cells. 1:500 should be sufficient for IF staining.
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FH Zeinab (Verified Customer) (08-19-2019) | It worked great
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FH Erica (Verified Customer) (05-15-2019) | Our lab has been using this antibody for IP, WB and IF for many years and it always worked well. I highly recommend this antibody, especially for IP for stress granules.
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FH Karthik (Verified Customer) (04-24-2019) | Upon induction of sodium arsenite stress in neurons G3BP1 positive stress granules formed in 90 minutes.Cells permeabilized with 0.2% triton for 10 minutes
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FH Tian (Verified Customer) (01-23-2019) | I used it for ICC and it worked Great.
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