Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 13057-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 13057-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13057-2-AP
WB result of G3BP1 antibody (13057-2-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G3BP1 transfected HEK-293 cells.
WB analysis using 13057-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13057-2-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13057-2-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13057-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 13057-2-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 13057-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 13057-2-AP
IP result of anti-G3BP1 (IP:13057-2-AP, 4ug; Detection:13057-2-AP 1:1000) with HEK-293 cells lysate 1040 ug.
IHC staining of human colon cancer using 13057-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 13057-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13057-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13057-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13057-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13057-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 13057-2-AP
Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP1 antibody (13057-2-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 13057-2-AP
Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP1 antibody (13057-2-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HeLa using 13057-2-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug G3BP1 Polyclonal antibody (13057-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

G3BP1 Polyclonal antibody

G3BP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 13057-2-AP

Publications(208)

Reviews (17)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Synonyms

ATP-dependent DNA helicase VIII, EC:3.6.4.12, G3BP, G3BP 1, G3BP-1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®555 CoraLite®594

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (208)
Protocols
Reviews (17)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	C6 cells, HEK-293 cells, human brain tissue, Neuro-2a cells, HeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, mouse kidney tissue, rat kidney tissue, mouse brain tissue, rat brain tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human colon cancer tissue, human breast cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	sodium arsenite treated HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 103 publications below
IHC	See 12 publications below
IF	See 149 publications below
IP	See 20 publications below
CoIP	See 8 publications below
RIP	See 4 publications below

Product Information

13057-2-AP targets G3BP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, chicken, drosophila melanogaster (fruit fly)
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	G3BP1 fusion protein Ag3728 Predict reactive species
Full Name	GTPase activating protein (SH3 domain) binding protein 1
Calculated Molecular Weight	466 aa, 52 kDa
Observed Molecular Weight	68 kDa
GenBank Accession Number	BC006997
Gene Symbol	G3BP1
Gene ID (NCBI)	10146
RRID	AB_2232034
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13283
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

Protocols

Product Specific Protocols
WB protocol for G3BP1 antibody 13057-2-AP	Download protocol
IHC protocol for G3BP1 antibody 13057-2-AP	Download protocol
IF protocol for G3BP1 antibody 13057-2-AP	Download protocol
IP protocol for G3BP1 antibody 13057-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human,chicken	IF,CoIP	Cell Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules Authors - Qinqin Cui View Article
human	IF	Nature m6A enhances the phase separation potential of mRNA. Authors - Ryan J Ries View Article
human	IP,IF	Science Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner. Authors - Youngdae Gwon View Article
human	IF	Cell ELAVL4, splicing, and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids. Authors - Kathryn R Bowles View Article
human	IF	Cell RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, Using Annexin A11 as a Molecular Tether. Authors - Ya-Cheng Liao View Article
human	IF	Cell Phase Separation of FUS Is Suppressed by Its Nuclear Import Receptor and Arginine Methylation. Authors - Mario Hofweber View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Xiaochen (Verified Customer) (11-11-2024)	Applications: Immunofluorescence G3BP1 Antibody Immunofluorescence validation ( dilution) in (Cat no:13057-2-AP)
FH Xiaochen (Verified Customer) (11-11-2024)	Applications: Immunofluorescence Cell Tissue Type: SH-SY5Y G3BP1 Antibody Immunofluorescence validation ( dilution) in SH-SY5Y (Cat no:13057-2-AP)
FH Roy (Verified Customer) (06-12-2024)	Works great on WB (1/1000 - Overnight 4°C) - Very beautiful IF staining in non stressed (diffuse staining) and Sodium Arsenite-mediated Stress induction (Punctate staining corresponding to stress granules) in HeLa cells (1/250 - 1h at RT). Applications: Western Blot, Immunofluorescence Primary Antibody Dilution: 1/1000 Cell Tissue Type: HeLa
FH Vinny (Verified Customer) (02-20-2024)	Good product. Applications: Immunofluorescence
FH Andrea (Verified Customer) (10-05-2023)	Good and strong signal. Applications: Western Blot
FH Tatyana (Verified Customer) (01-21-2023)	Used for IP and WB of GFP-tagged overexpressed human G3BP1 (HEK293 cells). Lysates were subjected to IP using GFP-Trap beads. WB was done using semi-dry transfer. Antibody was used in 4% milk in TBST at 1:1,000 dilution. It could be reused up to 3 times. As the image shows, the antibody can successfully detect both endogenous and OE protein. Applications: Immunofluorescence, Immunoprecipitation Primary Antibody Dilution: 1:1000 Cell Tissue Type: HEK293 G3BP1 Antibody Immunofluorescence,Immunoprecipitation validation (1:1000 dilution) in HEK293 (Cat no:13057-2-AP)
FH Tobias (Verified Customer) (10-25-2022)	Applications: Western Blot
FH Peter (Verified Customer) (10-24-2022)	Best G3BP1 antibody I've used for Western, IF and IP Applications: Western Blot, Immunofluorescence, Immunoprecipitation Primary Antibody Dilution: 1:1000 for WB. 1:200 for IF Cell Tissue Type: A549 and U2OS
FH Patryk (Verified Customer) (03-18-2021)	I used the antibody for immunofluorescence imaging to label stress granules induced by treating the cells with with 50µM sodium arsenite. Used the antibody at a dilution 1:100 overnight at 4°C. Worked perfectly well, strong and specific signal. I am very satisfied of this antibody and strongly recommend if for immunofluorescence. Applications: Immunofluorescence Primary Antibody Dilution: 1:100 Cell Tissue Type: HCT116 cells G3BP1 Antibody Immunofluorescence validation (1:100 dilution) in HCT116 cells (Cat no:13057-2-AP)
FH Kun (Verified Customer) (03-23-2020)	Very specific and sensitive Applications: Western Blot Primary Antibody Dilution: 1:1000
FH Biao (Verified Customer) (03-11-2020)	This antibody is very specific and good quality. Applications: Western Blot, Cell Tissue Type: hct-116
FH Joshua (Verified Customer) (12-28-2019)	PANC1 cells fixed in 4% paraformaldehdye. Bright localization to stress granules. Applications: Immunofluorescence, Primary Antibody Dilution: 1:500 Cell Tissue Type: human pancreatic adenocarcinoma cells G3BP1 Antibody Immunofluorescence, validation (1:500 dilution) in human pancreatic adenocarcinoma cells (Cat no:13057-2-AP)
FH Yuan (Verified Customer) (11-02-2019)	Very bright staining for stress granule on NaAsO2 treated Hela cells. 1:500 should be sufficient for IF staining. Applications: Immunofluorescence, Primary Antibody Dilution: 1:250 Cell Tissue Type: Hela G3BP1 Antibody Immunofluorescence, validation (1:250 dilution) in Hela (Cat no:13057-2-AP)
FH Zeinab (Verified Customer) (08-19-2019)	It worked great Applications: Immunofluorescence, Primary Antibody Dilution: 1:400
FH Erica (Verified Customer) (05-15-2019)	Our lab has been using this antibody for IP, WB and IF for many years and it always worked well. I highly recommend this antibody, especially for IP for stress granules. Applications: Western Blot, Immunofluorescence, Immunoprecipitation, Cell Tissue Type: HeLa; MEF
FH Karthik (Verified Customer) (04-24-2019)	Upon induction of sodium arsenite stress in neurons G3BP1 positive stress granules formed in 90 minutes.Cells permeabilized with 0.2% triton for 10 minutes Applications: Immunofluorescence, Primary Antibody Dilution: 1: 500 overnight at 4 degrees Cell Tissue Type: Primary motor neurons G3BP1 Antibody Immunofluorescence, validation (1: 500 overnight at 4 degrees dilution) in Primary motor neurons (Cat no:13057-2-AP)
FH Tian (Verified Customer) (01-23-2019)	I used it for ICC and it worked Great. Applications: Immunofluorescence, Primary Antibody Dilution: 1;100 Cell Tissue Type: NIH3T3