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G3BP1 Polyclonal antibody

G3BP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13057-2-AP

Synonyms

G3BP, G3BP 1, G3BP1, HDH VIII

Validation Data Gallery

  • WB analysis using 13057-2-AP

    WB analysis using 13057-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 13057-2-AP

    WB analysis of HEK-293 using 13057-2-AP

    WB result of G3BP1 antibody (13057-2-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G3BP1 transfected HEK-293 cells.

  • IHC staining of human testis using 13057-2-AP

    IHC staining of human testis using 13057-2-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 13057-2-AP

    IHC staining of human testis using 13057-2-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HEK-293 using 13057-2-AP

    IP experiment of HEK-293 using 13057-2-AP

    IP Result of anti-G3BP1 (IP:13057-2-AP, 3ug; Detection:13057-2-AP 1:1000) with HEK-293 cells lysate 2000ug.

  • IF Staining of HeLa using 13057-2-AP

    IF Staining of HeLa using 13057-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13057-2-AP (G3BP1 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 13057-2-AP

    FC experiment of HeLa using 13057-2-AP

    1X10^6 HeLa cells were stained with 0.20ug G3BP1 antibody (13057-2-AP, red) and control antibody (blue). Fixed with 90% MeOH.

Tested Applications

Positive WB detected inNeuro-2a cells, C6 cells, HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, MCF-7 cells, rat brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:300
Sample-dependent, check data in validation data gallery

Product Information

13057-2-AP targets G3BP1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen G3BP1 fusion protein Ag3728
Full Name GTPase activating protein (SH3 domain) binding protein 1
Calculated molecular weight 466 aa, 52 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC006997
Gene symbol G3BP1
Gene ID (NCBI) 10146
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

Protocols

Product Specific Protocols
WB protocol for G3BP1 antibody 13057-2-APDownload protocol
IHC protocol for G3BP1 antibody 13057-2-APDownload protocol
IF protocol for G3BP1 antibody 13057-2-APDownload protocol
IP protocol for G3BP1 antibody 13057-2-APDownload protocol
FC protocol for G3BP1 antibody 13057-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Hum Mol Genet

Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS.

Authors - Desiree M Baron
View Article
humanIF

Neuron

RNA Binding Antagonizes Neurotoxic Phase Transitions of TDP-43.

Authors - Jacob R Mann
View Article
IF

Neurochem Res

Missing in Action: Dysfunctional RNA Metabolism in Oligodendroglial Cells as a Contributor to Neurodegenerative Diseases?

Authors - Peter Hoch-Kraft
View Article
humanWB

Mol Cell

ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97.

Authors - Bo Wang
View Article
mouseWB

Am J Physiol Renal Physiol

Stress granules are formed in renal proximal tubular cells during metabolic stress and ischemic injury for cell survival.

Authors - Shixuan Wang
  • KD Validated
View Article
mouseIF

J Biol Chem

The RNA-binding protein FUS/TLS undergoes calcium-mediated nuclear egress during excitotoxic stress and is required for GRIA2 mRNA processing.

Authors - Maeve Tischbein
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Kun (Verified Customer) (03-23-2020)

Very specific and sensitive

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
FH

Biao (Verified Customer) (03-11-2020)

This antibody is very specific and good quality.

  • Applications: Western Blot,
  • Cell Tissue Type: hct-116
FH

Joshua (Verified Customer) (12-28-2019)

PANC1 cells fixed in 4% paraformaldehdye. Bright localization to stress granules.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: human pancreatic adenocarcinoma cells
G3BP1 Antibody Immunofluorescence, validation (1:500 dilution) in human pancreatic adenocarcinoma cells (Cat no:13057-2-AP)
FH

Yuan (Verified Customer) (11-02-2019)

Very bright staining for stress granule on NaAsO2 treated Hela cells. 1:500 should be sufficient for IF staining.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Hela
G3BP1 Antibody Immunofluorescence, validation (1:250 dilution) in Hela (Cat no:13057-2-AP)
FH

Zeinab (Verified Customer) (08-19-2019)

It worked great

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:400
FH

Erica (Verified Customer) (05-15-2019)

Our lab has been using this antibody for IP, WB and IF for many years and it always worked well. I highly recommend this antibody, especially for IP for stress granules.

  • Applications: Western Blot,Immunofluorescence,Immunoprecipitation,
  • Cell Tissue Type: HeLa; MEF
FH

Karthik (Verified Customer) (04-24-2019)

Upon induction of sodium arsenite stress in neurons G3BP1 positive stress granules formed in 90 minutes. Cells permeabilized with 0.2% triton for 10 minutes

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1: 500 overnight at 4 degrees
  • Cell Tissue Type: Primary motor neurons
G3BP1 Antibody Immunofluorescence, validation (1: 500 overnight at 4 degrees dilution) in Primary motor neurons (Cat no:13057-2-AP)
FH

Tian (Verified Customer) (01-23-2019)

I used it for ICC and it worked Great.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1;100
  • Cell Tissue Type: NIH3T3